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High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus
Journal article   Open access   Peer reviewed

High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

P.E. Bayer, P. Ruperao, A.S. Mason, J. Stiller, C-K.K. Chan, S. Hayashi, Y. Long, J. Meng, T. Sutton, P. Visendi, …
Theoretical and Applied Genetics, Vol.128(6), pp.1039-1047
2015
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Abstract

The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene–trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.4 Crop Science
3.4.96 QTL
Web Of Science research areas
Agronomy
Genetics & Heredity
Horticulture
Plant Sciences
ESI research areas
Agricultural Sciences
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