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How to develop and prove high-efficiency selection of ligands from oligonucleotide libraries: A universal framework for aptamers and DNA-Encoded small-molecule ligands
Journal article   Peer reviewed

How to develop and prove high-efficiency selection of ligands from oligonucleotide libraries: A universal framework for aptamers and DNA-Encoded small-molecule ligands

A.T.H. Le, S.M. Krylova, S.S. Beloborodov, T.Y. Wang, R. Hili, P.E. Johnson, F. Li, R.N. Veedu, S. Belyanskaya and S.N. Krylov
Analytical Chemistry, Vol.93(13), pp.5343-5354
2021
DOI: https://doi.org/10.1021/acs.analchem.1c00601
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Abstract

Screening molecular libraries for ligands capable of binding proteins is widely used for hit identification in the early drug discovery process. Oligonucleotide libraries provide a very high diversity of compounds, while the combination of the polymerase chain reaction and DNA sequencing allow the identification of ligands in low copy numbers selected from such libraries. Ligand selection from oligonucleotide libraries requires mixing the library with the target followed by the physical separation of the ligand–target complexes from the unbound library. Cumulatively, the low abundance of ligands in the library and the low efficiency of available separation methods necessitate multiple consecutive rounds of partitioning. Multiple rounds of inefficient partitioning make the selection process ineffective and prone to failures. There are continuing efforts to develop a separation method capable of reliably generating a pure pool of ligands in a single round of partitioning; however, none of the proposed methods for single-round selection have been universally adopted. Our analysis revealed that the developers’ efforts are disconnected from each other and hindered by the lack of quantitative criteria of selection quality assessment. Here, we present a formalism that describes single-round selection mathematically and provides parameters for quantitative characterization of selection quality. We use this formalism to define a universal strategy for development and validation of single-round selection methods. Finally, we analyze the existing partitioning methods, the published single-round selection reports, and some pertinent practical considerations through the prism of this formalism. This formalism is not an experimental protocol but a framework for correct development of experimental protocols. While single-round selection is not a goal by itself and may not always suffice selection of good-quality ligands, our work will help developers of highly efficient selection approaches to consolidate their efforts under an umbrella of universal quantitative criteria of method development and assessment.

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2 Chemistry
2.145 Biosensors
2.145.243 Nanobiosensors
Web Of Science research areas
Chemistry, Analytical
ESI research areas
Chemistry
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