Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia

S. Guo; D. Wakeham; H.J.M. Brouwers; R.N. Cobbold; S. Abraham; J.L. Mollinger; J.R. Johnson; T.A. Chapman; D.M. Gordon; V.R. Barrs; D.J. Trott

doi:10.1016/j.micinf.2014.12.016

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Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia

Journal article

Peer reviewed

Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia

S. Guo, D. Wakeham, H.J.M. Brouwers, R.N. Cobbold, S. Abraham, J.L. Mollinger, J.R. Johnson, T.A. Chapman, D.M. Gordon, V.R. Barrs, …

Microbes and Infection, Vol.17(4), pp.266-274

2015

DOI: https://doi.org/10.1016/j.micinf.2014.12.016

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Abstract

Phylogenetic group D extraintestinal pathogenic Escherichia coli (ExPEC), including O15:K52:H1 and clonal group A, have spread globally and become fluoroquinolone-resistant. Here we investigated the role of canine feces as a reservoir of these (and other) human-associated ExPEC and their potential as canine pathogens. We characterized and compared fluoroquinolone-resistant E. coli isolates originally identified as phylogenetic group D from either the feces of hospitalized dogs (n = 67; 14 dogs) or extraintestinal infections (n = 53; 33 dogs). Isolates underwent phylogenetic grouping, random amplified polymorphic DNA (RAPD) analysis, virulence genotyping, resistance genotyping, human-associated ExPEC O-typing, and multi-locus sequence typing. Five of seven human-associated sequence types (STs) exhibited ExPEC-associated O-types, and appeared in separate RAPD clusters. The largest subgroup (16 fecal, 26 clinical isolates) were ST354 (phylogroup F) isolates. ST420 (phylogroup B2); O1-ST38, O15:K52:H1-ST393, and O15:K1-ST130 (phylogroup D); and O7-ST457, and O1-ST648 (phylogroup F) were also identified. Three ST-specific RAPD sub-clusters (ST354, ST393, and ST457) contained closely related isolates from both fecal or clinical sources. Genes encoding CTX-M and AmpC β-lactamases were identified in isolates from five STs. Major human-associated fluoroquinolone-resistant ± extended-spectrum cephalosporin-resistant ExPEC of public health importance may be carried in dog feces and cause extraintestinal infections in some dogs.

Details

Title: Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia
Authors/Creators: S. Guo (Author/Creator)
D. Wakeham (Author/Creator)
H.J.M. Brouwers (Author/Creator)
R.N. Cobbold (Author/Creator)
S. Abraham (Author/Creator)
J.L. Mollinger (Author/Creator)
J.R. Johnson (Author/Creator)
T.A. Chapman (Author/Creator)
D.M. Gordon (Author/Creator)
V.R. Barrs (Author/Creator)
D.J. Trott (Author/Creator)
Publication Details: Microbes and Infection, Vol.17(4), pp.266-274
Publisher: Elsevier Masson SAS
Identifiers: 991005542985507891
Copyright: © 2015 Institut Pasteur.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.23 Antibiotics & Antimicrobials; 1.23.146 Antimicrobial Resistance
Web Of Science research areas: Immunology; Infectious Diseases; Microbiology
ESI research areas: Immunology