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Published (Version of Record)CC BY-NC-SA V4.0, Open Access
Journal article
Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons
Scientific Reports, Vol.1, Article 140
2011
Abstract
Transport of mRNAs to diverse neuronal locations via RNA granules serves an important function in regulating protein synthesis within restricted sub-cellular domains. We recently detected the Huntington's disease protein huntingtin (Htt) in dendritic RNA granules; however, the functional significance of this localization is not known. Here we report that Htt and the huntingtin-associated protein 1 (HAP1) are co-localized with the microtubule motor proteins, the KIF5A kinesin and dynein, during dendritic transport of β-actin mRNA. Live cell imaging demonstrated that β-actin mRNA is associated with Htt, HAP1, and dynein intermediate chain in cultured neurons. Reduction in the levels of Htt, HAP1, KIF5A, and dynein heavy chain by lentiviral-based shRNAs resulted in a reduction in the transport of β-actin mRNA. These findings support a role for Htt in participating in the mRNA transport machinery that also contains HAP1, KIF5A, and dynein.
Details
- Title
- Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons
- Authors/Creators
- B. Ma (Author/Creator) - New York UniversityJ.N. Savas (Author/Creator) - New York UniversityM-S Yu (Author/Creator) - New York UniversityB.P. Culver (Author/Creator) - New York UniversityM.V. Chao (Author/Creator) - New York UniversityN. Tanese (Author/Creator) - New York University
- Publication Details
- Scientific Reports, Vol.1, Article 140
- Publisher
- Nature Publishing Group
- Identifiers
- 991005546006807891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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