Journal article
Hydrogen Peroxide Biosensors Based on the Immobilization of Hemoglobin on Amino-Silane Magnetic Nanoparticle-Contained Carbon Paste Electrodes
Sensor Letters, Vol.8(6), pp.864-870
2010
Abstract
Amino-silane magnetic nanoparticles (A-SMNPs) were mixed with graphite powder to construct A-SMNPs-contained carbon paste electrodes (CPE). Hemoglobin (Hb) was then immobilized on the electrode surface to form Hb/A-SMNPs/CPE biosensors. The biosensor was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The results indicated that Hb was stably immobilized on the electrode and exhibit excellent electrocatalytic activity to the reduction of hydrogen peroxide without the aid of an electron mediator. When used for amperometric measurement of H2O2, the prepared biosensor displayed good sensitivity, selectivity and stability. A linear detection range of H2O2 from 1.0 mu mol L-1 to 2.0 mmol L-1 and a detection limit of 0.27 mu mol L-1 (S/N = 3) was obtained. The apparent Michaelis-Menten constant was calculated to be 0.85 mmol L-1, indicating that the immobilized Hb had high enzymatic activity to H2O2.
Details
- Title
- Hydrogen Peroxide Biosensors Based on the Immobilization of Hemoglobin on Amino-Silane Magnetic Nanoparticle-Contained Carbon Paste Electrodes
- Authors/Creators
- Y-M Huang (Author/Creator)H-L Zhang (Author/Creator)A-M Yu (Author/Creator)Y. Liu (Author/Creator)Y-W Wu (Author/Creator)
- Publication Details
- Sensor Letters, Vol.8(6), pp.864-870
- Publisher
- American Scientific Publishers
- Identifiers
- 991005540469707891
- Copyright
- (c) American Scientific Publishers
- Murdoch Affiliation
- School of Chemical and Mathematical Science
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.145 Biosensors
- 2.145.177 Nanomaterial Sensors
- Web Of Science research areas
- Chemistry, Analytical
- Electrochemistry
- Instruments & Instrumentation
- Physics, Applied
- ESI research areas
- Chemistry