Identification and Fine Mapping of a White Husk Gene in Barley (Hordeum vulgare L.)

W. Hua; X-Q Zhang; J. Zhu; Y. Shang; J. Wang; Q. Jia; Q. Zhang; J. Yang; C. Li

doi:10.1371/journal.pone.0152128

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Identification and Fine Mapping of a White Husk Gene in Barley (Hordeum vulgare L.)

Journal article

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Identification and Fine Mapping of a White Husk Gene in Barley (Hordeum vulgare L.)

W. Hua, X-Q Zhang, J. Zhu, Y. Shang, J. Wang, Q. Jia, Q. Zhang, J. Yang and C. Li

PLoS ONE, Vol.11(3), e0152128

2016

DOI: https://doi.org/10.1371/journal.pone.0152128

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Abstract

Barley is the only crop in the Poaceae family with adhering husks at maturity. The color of husk at barely development stage could influence the agronomic traits and malting qualities of grains. A barley mutant with a white husk was discovered from the malting barley cultivar Supi 3 and designated wh (white husk). Morphological changes and the genetics of white husk barley were investigated. Husks of the mutant were white at the heading and flowering stages but yellowed at maturity. The diastatic power and α-amino nitrogen contents also significantly increased in wh mutant. Transmission electron microscopy examination showed abnormal chloroplast development in the mutant. Genetic analysis of F2 and BC1F1 populations developed from a cross of wh and Yangnongpi 5 (green husk) showed that the white husk was controlled by a single recessive gene (wh). The wh gene was initially mapped between 49.64 and 51.77 cM on chromosome 3H, which is syntenic with rice chromosome 1 where a white husk gene wlp1 has been isolated. The barley orthologous gene of wlp1 was sequenced from both parents and a 688 bp deletion identified in the wh mutant. We further fine-mapped the wh gene between SSR markers Bmac0067 and Bmag0508a with distances of 0.36 cM and 0.27 cM in an F2 population with 1115 individuals of white husk. However, the wlp1 orthologous gene was mapped outside the interval. New candidate genes were identified based on the barley genome sequence.

Details

Title: Identification and Fine Mapping of a White Husk Gene in Barley (Hordeum vulgare L.)
Authors/Creators: W. Hua (Author/Creator) - ZheJiang Academy of Agricultural Sciences
X-Q Zhang (Author/Creator) - Murdoch University
J. Zhu (Author/Creator) - ZheJiang Academy of Agricultural Sciences
Y. Shang (Author/Creator) - ZheJiang Academy of Agricultural Sciences
J. Wang (Author/Creator) - ZheJiang Academy of Agricultural Sciences
Q. Jia (Author/Creator) - ZheJiang Academy of Agricultural Sciences
Q. Zhang (Author/Creator) - Australian Export Grains Innovation Centre
J. Yang (Author/Creator) - ZheJiang Academy of Agricultural Sciences
C. Li (Author/Creator) - Murdoch University
Publication Details: PLoS ONE, Vol.11(3), e0152128
Publisher: Public Library of Science
Identifiers: 991005540158507891
Copyright: © 2016 Hua et al.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science