Identification and characterization of fast- and slow-growing root nodule bacteria from South-Western Australian soils able to nodulate Acacia saligna

N.D.S. Marsudi; A.R. Glenn; M.J. Dilworth

doi:10.1016/S0038-0717(99)00032-2

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Identification and characterization of fast- and slow-growing root nodule bacteria from South-Western Australian soils able to nodulate Acacia saligna

Journal article

Peer reviewed

Identification and characterization of fast- and slow-growing root nodule bacteria from South-Western Australian soils able to nodulate Acacia saligna

N.D.S. Marsudi, A.R. Glenn and M.J. Dilworth

Soil Biology and Biochemistry, Vol.31(9), pp.1229-1238

1999

DOI: https://doi.org/10.1016/S0038-0717(99)00032-2

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Abstract

A total of 133 root nodule bacterial strains were isolated from nodules of Acacia saligna growing in soils from nine geographically separate locations in South-Western Australia; 40 were characterized on the basis of their growth and physiology and 20 by 16 S rRNA sequence analysis. Thirty-nine strains were fast-growing rhizobia, and 94 slow-growing bradyrhizobia. The latter were essentially acid-tolerant, alkali-sensitive and salt-sensitive, while the former varied in acid-tolerance and were alkali- and salt-tolerant. In addition many of the rhizobia grew at 37C whereas few of the bradyrhizobia could. The fastgrowing strains utilized disaccharides whereas the slow-growing bradyrhizobia did not. Using PCR a 260-264 bp segment of the 16 S rRNA gene was amplified and then sequenced. The fast-growing rhizobia can be divided into at least two groups - one showing similarity to Rhizobium leguminosarum biovar phaseoli and the other to R. tropici. The slow-growers showed relationships to either Bradyrhizobium japonicum or Bradyrhizobium spp. (Lupinus).

Details

Title: Identification and characterization of fast- and slow-growing root nodule bacteria from South-Western Australian soils able to nodulate Acacia saligna
Authors/Creators: N.D.S. Marsudi (Author/Creator)
A.R. Glenn (Author/Creator)
M.J. Dilworth (Author/Creator)
Publication Details: Soil Biology and Biochemistry, Vol.31(9), pp.1229-1238
Publisher: Elsevier BV
Identifiers: 991005543745707891
Murdoch Affiliation: Centre for Rhizobium Studies; School of Biological Sciences and Biotechnology
Language: English
Resource Type: Journal article

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Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.892 Rhizobium-Legume Symbiosis
Web Of Science research areas: Soil Science
ESI research areas: Agricultural Sciences