Journal article
Identification and comparative analysis of differential gene expression in soybean leaf tissue under drought and flooding stress revealed by RNA-Seq
Frontiers in Plant Science, Vol.7, Art. 01044
2016
Abstract
Drought and flooding are two major causes of severe yield loss in soybean worldwide. A lack of knowledge of the molecular mechanisms involved in drought and flood stress has been a limiting factor for the effective management of soybeans; therefore, it is imperative to assess the expression of genes involved in response to flood and drought stress. In this study, differentially expressed genes (DEGs) under drought and flooding conditions were investigated using Illumina RNA-Seq transcriptome profiling. A total of 2724 and 3498 DEGs were identified under drought and flooding treatments, respectively. These genes comprise 289 Transcription Factors (TFs) representing Basic Helix-loop Helix (bHLH), Ethylene Response Factors (ERFs), myeloblastosis (MYB), No apical meristem (NAC), and WRKY amino acid motif (WRKY) type major families known to be involved in the mechanism of stress tolerance. The expression of photosynthesis and chlorophyll synthesis related genes were significantly reduced under both types of stresses, which limit the metabolic processes and thus help prolong survival under extreme conditions. However, cell wall synthesis related genes were up-regulated under drought stress and down-regulated under flooding stress. Transcript profiles involved in the starch and sugar metabolism pathways were also affected under both stress conditions. The changes in expression of genes involved in regulating the flux of cell wall precursors and starch/sugar content can serve as an adaptive mechanism for soybean survival under stress conditions. This study has revealed the involvement of TFs, transporters, and photosynthetic genes, and has also given a glimpse of hormonal cross talk under the extreme water regimes, which will aid as an important resource for soybean crop improvement.
Details
- Title
- Identification and comparative analysis of differential gene expression in soybean leaf tissue under drought and flooding stress revealed by RNA-Seq
- Authors/Creators
- W. Chen (Author/Creator) - University of MissouriQ. Yao (Author/Creator) - University of MissouriG.B. Patil (Author/Creator) - University of MissouriG. Agarwal (Author/Creator) - Crops Research InstituteR.K. Deshmukh (Author/Creator) - University of MissouriL. Lin (Author/Creator) - University of MissouriB. Wang (Author/Creator) - Shanghai Jiao Tong UniversityY. Wang (Author/Creator) - University of MissouriS.J. Prince (Author/Creator) - University of MissouriL. Song (Author/Creator) - University of MissouriD. Xu (Author/Creator) - University of MissouriY.C. An (Author/Creator)B. Valliyodan (Author/Creator) - University of MissouriR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsH.T. Nguyen (Author/Creator) - University of Missouri
- Publication Details
- Frontiers in Plant Science, Vol.7, Art. 01044
- Publisher
- Frontiers
- Identifiers
- 991005541228007891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
45 File views/ downloads
57 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science