Identification and expression analysis of Phosphate Transporter 1 (PHT1) genes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)

Benjamin J. Nestor; Toby Bird; Anita A. Severn-Ellis; Philipp E. Bayer; Kosala Ranathunge; M.  Asaduzzaman Prodhan; Maheshi Dassanayake; Jacqueline Batley; David Edwards; Hans Lambers; Patrick M. Finnegan

doi:10.1111/pce.15088

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Identification and expression analysis of Phosphate Transporter 1 (PHT1) genes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)

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Identification and expression analysis of Phosphate Transporter 1 (PHT1) genes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)

Benjamin J. Nestor, Toby Bird, Anita A. Severn-Ellis, Philipp E. Bayer, Kosala Ranathunge, M. Asaduzzaman Prodhan, Maheshi Dassanayake, Jacqueline Batley, David Edwards, Hans Lambers, …

Plant, cell and environment, Vol.47(12), pp.5021-5038

2024

DOI: https://doi.org/10.1111/pce.15088

PMID: 39136390

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Abstract

crop production

fertilisers

gene expression analysis

inorganic phosphate transporters

nanopore sequencing

phylogenetic trees

plant genome

tertiary protein structure

Heavy and costly use of phosphorus (P) fertiliser is often needed to achieve high crop yields, but only a small amount of applied P fertiliser is available to most crop plants. Hakea prostrata (Proteaceae) is endemic to the P-impoverished landscape of southwest Australia and has several P-saving traits. We identified 16 members of the Phosphate Transporter 1 (PHT1) gene family (HpPHT1;1-HpPHT1;12d) in a long-read genome assembly of H. prostrata. Based on phylogenetics, sequence structure and expression patterns, we classified HpPHT1;1 as potentially involved in Pi uptake from soil and HpPHT1;8 and HpPHT1;9 as potentially involved in Pi uptake and root-to-shoot translocation. Three genes, HpPHT1;4, HpPHT1;6 and HpPHT1;8, lacked regulatory PHR1-binding sites (P1BS) in the promoter regions. Available expression data for HpPHT1;6 and HpPHT1;8 indicated they are not responsive to changes in P supply, potentially contributing to the high P sensitivity of H. prostrata. We also discovered a Proteaceae-specific clade of closely-spaced PHT1 genes that lacked conserved genetic architecture among genera, indicating an evolutionary hot spot within the genome. Overall, the genome assembly of H. prostrata provides a much-needed foundation for understanding the genetic mechanisms of novel adaptations to low P soils in southwest Australian plants.

Details

Title: Identification and expression analysis of Phosphate Transporter 1 (PHT1) genes in the highly phosphorus-use-efficient Hakea prostrata (Proteaceae)
Authors/Creators: Benjamin J. Nestor - The University of Western Australia
Toby Bird - School of Biological Sciences, University of Western Australia, Perth, WA, Australia
Anita A. Severn-Ellis - The University of Western Australia
Philipp E. Bayer - The University of Western Australia
Kosala Ranathunge - The University of Western Australia
M. Asaduzzaman Prodhan - Centre for Applied Bioinformatics, School of Biological Sciences, The University of Western Australia, Perth, WA, Australia
Maheshi Dassanayake - Louisiana State University
Jacqueline Batley - The University of Western Australia
David Edwards - The University of Western Australia
Hans Lambers - The University of Western Australia
Patrick M. Finnegan - The University of Western Australia
Publication Details: Plant, cell and environment, Vol.47(12), pp.5021-5038
Publisher: John Wiley & Sons Ltd.
Number of pages: 18
Grant note: Government of Western Australia (501100011025) Australian University Librarians DP140100148 / Australian Research Council (501100000923) University of Western Australia (http://data.elsevier.com/vocabulary/SciValFunders/501100001801) Australian Government (100015539) DP140100148; LP0776252; DP200101013 / Australian Research Council (http://data.elsevier.com/vocabulary/SciValFunders/501100000923) DP200101013 / Australian Research Council (501100000923) LP0776252 / Australian Research Council (501100000923) Australian Government (http://data.elsevier.com/vocabulary/SciValFunders/100015539) Australian Research Council (http://data.elsevier.com/vocabulary/SciValFunders/501100000923)
Identifiers: 991005838680007891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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#12 Responsible Consumption & Production

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.45 Soil Science; 3.45.473 Soil Phosphorus Dynamics
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science