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Open Access CC BY-NC-ND V4.0
Journal article
Identification and functional validation of five cytochrome P450 monooxygenase genes conferring cyantraniliprole resistance in Bemisia tabaci
Journal of Integrative Agriculture, In Press, Journal Pre-proof
2026
Abstract
The whitefly, Bemisia tabaci, is a major agricultural pest that has developed resistance to a broad range of insecticides. Despite the promising efficacy of cyantraniliprole (CYA) against B. tabaci, medium to high levels of resistance have emerged after prolonged field use. However, the mechanisms driving CYA resistance remain poorly understood. In this study, four B. tabaci strains exhibiting 24.9-fold to 28.9-fold resistance ratio to CYA were investigated. Synergist assays and enzyme activity measurements indicated cytochrome P450 enzymes contribute to this resistance. RNA sequencing and RT-qPCR analysis identified five P450 genes (CYP305H2, CYP6EM1, CYP3133D3, CYP3133D5, and CYP3133E2) as significantly overexpressed in resistant strains. Targeted silencing of these genes led to a 22.3% to 50.3% increase in CYA toxicity. The metabolic rates of these P450 enzymes against CYA were 2.5- to 6.0-fold higher than that in the control group within two hours. These results provide new insights into the molecular basis of CYA resistance in B. tabaci and highlight the pivotal role of cytochrome P450 enzymes in metabolic adaptation to diamide insecticides.
Details
- Title
- Identification and functional validation of five cytochrome P450 monooxygenase genes conferring cyantraniliprole resistance in Bemisia tabaci
- Authors/Creators
- Xiaolan Liu - Qingdao Agricultural UniversityZhuang Zhang - Qingdao Agricultural UniversityKaixin Li - Qingdao Agricultural UniversityZanrong Wen - Qingdao Agricultural UniversityWei Xu - Murdoch UniversityHuiwen Tan - Qingdao Agricultural UniversityXichao Hu - Qingdao Agricultural UniversityLei Guo - Qingdao Agricultural University
- Publication Details
- Journal of Integrative Agriculture, In Press, Journal Pre-proof
- Publisher
- Elsevier B.V
- Identifiers
- 991005884925107891
- Murdoch Affiliation
- School of Agricultural Sciences; Centre for Sustainable Farming Systems
- Language
- English
- Resource Type
- Journal article
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