Journal article
Identification of Sources of Resistance toPhoma medicaginisIsolates inMedicago truncatulaSARDI Core Collection Accessions, and Multigene Differentiation of Isolates
Phytopathology, Vol.96(12), pp.1330-1336
12/2006
Abstract
Phoma medicaginis is a necrotrophic fungal pathogen, commonly found infecting the annual medic Medicago truncatula. To differentiate eight P. medicaginis isolates, five gene regions were examined: actin, β-tubulin, calmodulin, translation elongation factor I-α (EF-1α), and the internal transcribed spacer ribosomal DNA. Sequence comparisons showed that specimens isolated from M. truncatula in Western Australia formed a group that was consistently different from, but allied to, a P. medicaginis var. medicaginis type specimen. EP-1α contained a hypervariable 55-bp repeat unit, which forms the basis of a rapid polymerase chain reaction-based method of reliably distinguishing isolates. Characterization of three isolates showed that all exhibited a narrow host range. causing disease only in M. sativa and M. truncatula among eight commonly cultivated legume species sampled. Infection of 86 M. truncatula single-seeded accessions showed a continuous distribution in disease phenotypes, with the majority of accessions susceptible. On a 1-to-5 disease reaction scale increasing in severity, individual fungal isolates showed means of 2.6 to 3.2. and scores ranged from 1 to 4.8 among accessions. The results presented here suggest that M. truncatula harbors specific and diverse sources of resistance to individual P. medicaginis genotypes.
Details
- Title
- Identification of Sources of Resistance toPhoma medicaginisIsolates inMedicago truncatulaSARDI Core Collection Accessions, and Multigene Differentiation of Isolates
- Authors/Creators
- S.R. Ellwood (Author/Creator)L.G. Kamphuis (Author/Creator)R.P. Oliver (Author/Creator)
- Publication Details
- Phytopathology, Vol.96(12), pp.1330-1336
- Publisher
- The American Phytopathological Society
- Identifiers
- 991005545511807891
- Copyright
- © 2006 The American Phytopathological Society.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; Australian Centre for Necrotrophic Fungal Pathogens
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.636 Fungal Plant Pathogens
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science