Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides

J.A.T. Morgan; S.W. Corley; L.A. Jackson; A.E. Lew-Tabor; P.M. Moolhuijzen; N.N. Jonsson

doi:10.1016/j.ijpara.2008.12.006

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Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides

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Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides

J.A.T. Morgan, S.W. Corley, L.A. Jackson, A.E. Lew-Tabor, P.M. Moolhuijzen and N.N. Jonsson

International Journal for Parasitology, Vol.39(7), pp.775-779

2009

DOI: https://doi.org/10.1016/j.ijpara.2008.12.006

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Abstract

Resistance against synthetic pyrethroid (SP) products for the control of cattle ticks in Australia was detected in the field in 1984, within a very short time of commercial introduction. We have identified a mutation in the domain II S4-5 linker of the para-sodium channel that is associated with resistance to SPs in the cattle tick Rhipicephalus (Boophilus) microplus from Australia. The cytosine to adenine mutation at position 190 in the R. microplus sequence AF134216, results in an amino acid substitution from leucine in the susceptible strain to isoleucine in the resistant strain. A similar mutation has been shown to confer SP resistance in the whitefly, Bemisia tabaci, but has not been described previously in ticks. A diagnostic quantitative PCR assay has been developed using allele-specific Taqman® minor groove-binding (MGB) probes. Using the assay to screen field and laboratory populations of ticks showed that homozygote allelic frequencies correlated highly with the survival percentage at the discriminating concentration of cypermethrin.

Details

Title: Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides
Authors/Creators: J.A.T. Morgan (Author/Creator) - Cooperative Research Centre for Beef Genetic Technologies
S.W. Corley (Author/Creator) - Cooperative Research Centre for Beef Genetic Technologies
L.A. Jackson (Author/Creator) - Cooperative Research Centre for Beef Genetic Technologies
A.E. Lew-Tabor (Author/Creator) - Murdoch University
P.M. Moolhuijzen (Author/Creator) - Murdoch University
N.N. Jonsson (Author/Creator) - Cooperative Research Centre for Beef Genetic Technologies
Publication Details: International Journal for Parasitology, Vol.39(7), pp.775-779
Publisher: Elsevier BV
Identifiers: 991005544011307891
Copyright: © 2009 Australian Society for Parasitology Inc
Murdoch Affiliation: Centre for Comparative Genomics
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.258 Zoonotic Diseases; 1.258.227 Tick-borne Pathogens
Web Of Science research areas: Parasitology
ESI research areas: Microbiology