Identification of genes controlling compatible and incompatible reactions of pearl millet (Pennisetum glaucum) against blast (Magnaporthe grisea) pathogen through RNA-Seq

Shweta Singh; Rajan Sharma; Thirunavukkarasu Nepolean; Spurthi N Nayak; Bheemavarapu Pushpavathi; Aamir W Khan; Rakesh K Srivastava; Rajeev K Varshney

doi:10.3389/fpls.2022.981295

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Identification of genes controlling compatible and incompatible reactions of pearl millet (Pennisetum glaucum) against blast (Magnaporthe grisea) pathogen through RNA-Seq

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Identification of genes controlling compatible and incompatible reactions of pearl millet (Pennisetum glaucum) against blast (Magnaporthe grisea) pathogen through RNA-Seq

Shweta Singh, Rajan Sharma, Thirunavukkarasu Nepolean, Spurthi N Nayak, Bheemavarapu Pushpavathi, Aamir W Khan, Rakesh K Srivastava and Rajeev K Varshney

Frontiers in plant science, Vol.13, 981295

2022

DOI: https://doi.org/10.3389/fpls.2022.981295

PMCID: PMC9544386

PMID: 36212352

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Abstract

Blast [Magnaporthe grisea (Herbert) Barr] is an economically important disease in Asian pearl millet production ecologies. The recurrent occurrence of blast in the past one decade has caused enormous strain on grain and forage production. Identification of resistance genes is an important step to develop durable varieties. The present study is the first attempt to use RNA-Seq to investigate the transcript dynamics in a pearl millet inbred ICMB 93333, which had a unique differential reaction to two isolates—Pg 45 (avirulent) and Pg 174 (virulent) of M. grisea. The inbred was inoculated by both isolates and samples taken at six different time intervals for genome-wide RNA-Seq experiment. The transcriptome results revealed the differential expression of more than 2,300 genes. The time-specific comparison showed activation or repression of specific genes in various pathways. Genes and transcriptions factors related to pathogenesis-related proteins, reactive oxygen species generating and its scavenging genes, cell wall defense, primary and secondary metabolic pathways, and signaling pathways were identified by comparing the host-plant compatible and incompatible interactions. The genes identified from this experiment could be useful to understand the host-plant resistance and design novel strategies to manage blast disease in pearl millet.

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Title: Identification of genes controlling compatible and incompatible reactions of pearl millet (Pennisetum glaucum) against blast (Magnaporthe grisea) pathogen through RNA-Seq
Authors/Creators: Shweta Singh
Rajan Sharma
Thirunavukkarasu Nepolean
Spurthi N Nayak
Bheemavarapu Pushpavathi
Aamir W Khan
Rakesh K Srivastava
Rajeev K Varshney
Publication Details: Frontiers in plant science, Vol.13, 981295
Publisher: Frontiers Media S.A.
Identifiers: 991005621241207891
Copyright: © 2022 Singh, Sharma, Nepolean, Nayak, Pushpavathi, Khan, Srivastava and Varshney.
Murdoch Affiliation: Food Futures Institute
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.419 Jasmonic Acid
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science