Journal article
Identification of low Ca2+stress-induced embryo apoptosis response genes in Arachis hypogaea by SSH-associated library lift (SSHaLL)
Plant Biotechnology Journal, Vol.14(2), pp.682-698
2015
Abstract
Calcium is a universal signal in the regulation of wide aspects in biology, but few are known about the function of calcium in the control of early embryo development. Ca2+ deficiency in soil induces early embryo abortion in peanut, producing empty pods, which is a general problem; however, the underlying mechanism remains unclear. In this study, embryo abortion was characterized to be caused by apoptosis marked with cell wall degradation. Using a method of SSH cDNA libraries associated with library lift (SSHaLL), 62 differentially expressed genes were isolated from young peanut embryos. These genes were classified to be stress responses, catabolic process, carbohydrate and lipid metabolism, embryo morphogenesis, regulation, etc. The cell retardation with cell wall degradation was caused by up-regulated cell wall hydrolases and down-regulated cellular synthases genes. HsfA4a, which was characterized to be important to embryo development, was significantly down-regulated under Ca2+-deficient conditions from 15 days after pegging (DAP) to 30 DAP. Two AhCYP707A4 genes, encoding abscisic acid (ABA) 8′-hydroxylases, key enzymes for ABA catabolism, were up-regulated by 21-fold under Ca2+-deficient conditions upstream of HsfA4a, reducing the ABA level in early embryos. Over-expression of AhCYP707A4 in Nicotiana benthamiana showed a phenotype of low ABA content with high numbers of aborted embryos, small pods and less seeds, which confirms that AhCYP707A4 is a key player in regulation of Ca2+ deficiency-induced embryo abortion via ABA-mediated apoptosis. The results elucidated the mechanism of low Ca2+-induced embryo abortion and described the method for other fields of study.
Details
- Title
- Identification of low Ca2+stress-induced embryo apoptosis response genes in Arachis hypogaea by SSH-associated library lift (SSHaLL)
- Authors/Creators
- H. Chen (Author/Creator)C. Zhang (Author/Creator)T.C. Cai (Author/Creator) - Fujian Agriculture and Forestry UniversityY. Deng (Author/Creator) - Fujian Agriculture and Forestry UniversityS. Zhou (Author/Creator)Y. Zheng (Author/Creator) - Fujian Agriculture and Forestry UniversityS. Ma (Author/Creator)R. Tang (Author/Creator)R.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsW. Zhuang (Author/Creator) - Fujian Agriculture and Forestry University
- Publication Details
- Plant Biotechnology Journal, Vol.14(2), pp.682-698
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005539996107891
- Copyright
- © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
28 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.1454 PCR Techniques
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Plant Sciences
- ESI research areas
- Plant & Animal Science