Journal article
Identification of microRNAs and their gene targets in cytoplasmic male sterile and fertile maintainer lines of pigeonpea
Planta, Vol.253(2), Art. 59
2021
Abstract
Exploitation of hybrid vigor using CMS technology has delivered nearly 50% yield gain in pigeonpea. Among various sterility-inducing cytoplasms (A1–A9) reported so far in pigeonpea, A2 and A4 are the two major sources that facilitate hybrid seed production. Recent evidence suggests involvement of micro RNA in vast array of biological processes including plant reproductive development. In pigeonpea, information about the miRNAs is insufficient. In view of this, we sequenced six small RNA libraries of CMS line UPAS 120A and isogenic fertile line UPAS 120B using Illumina technology. Results revealed 316 miRNAs including 248 known and 68 novel types. A total of 637 gene targets were predicted for known miRNAs, while 324 genes were associated with novel miRNAs. Degradome analysis revealed 77 gene targets of predicted miRNAs, which included a variety of transcription factors playing key roles in plant reproduction such as F-box family proteins, apetala 2, auxin response factors, ethylene-responsive factors, homeodomain-leucine zipper proteins etc. Differential expression of both known and novel miRNAs implied roles for both conserved as well as species-specific players. We also obtained several miRNA families such as miR156, miR159, miR167 that are known to influence crucial aspects of plant fertility. Gene ontology and pathway level analyses of the target genes showed their possible implications for crucial events during male reproductive development such as tapetal degeneration, pollen wall formation, retrograde signaling etc. To the best of our knowledge, present study is first to combine deep sequencing of small RNA and degradome for elucidating the role of miRNAs in flower and male reproductive development in pigeonpea.
Details
- Title
- Identification of microRNAs and their gene targets in cytoplasmic male sterile and fertile maintainer lines of pigeonpea
- Authors/Creators
- A. Bohra (Author/Creator) - Indian Institute of Pulses ResearchP. Gandham (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA. Rathore (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsV. Thakur (Author/Creator) - University of HyderabadR.K. Saxena (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsS.J.S. Naik (Author/Creator) - Indian Institute of Pulses ResearchR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsN.P. Singh (Author/Creator) - Indian Institute of Pulses Research
- Publication Details
- Planta, Vol.253(2), Art. 59
- Publisher
- Springer-Verlag
- Identifiers
- 991005540402707891
- Copyright
- © 2021 Springer Nature Switzerland AG.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
56 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.159 Arabidopsis
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science