Journal article
Identification of novel yellow fever Class II epitopes in YF-17D vaccinees
Viruses, Vol.12(11), Article 1300
2020
Abstract
Yellow fever virus (YFV) is a mosquito-borne member of the genus flavivirus, including other important human-pathogenic viruses, such as dengue, Japanese encephalitis, and Zika. Herein, we report identifying 129 YFV Class II epitopes in donors vaccinated with the live attenuated YFV vaccine (YFV-17D). A total of 1156 peptides predicted to bind 17 different common HLA-DRB1 allelic variants were tested using IFNγ ELISPOT assays in vitro re-stimulated peripheral blood mononuclear cells from twenty-six vaccinees. Overall, we detected responses against 215 YFV epitopes. We found that the capsid and envelope proteins, as well as the non-structural (NS) proteins NS3 and NS5, were the most targeted proteins by CD4+ T cells from YF-VAX vaccinated donors. In addition, we designed and validated by flow cytometry a CD4+ mega pool (MP) composed of structural and non-structural epitopes in an independent cohort of vaccinated donors. Overall, this study provides a comprehensive prediction and validation of YFV epitopes in a cohort of YF-17D vaccinated individuals. With the design of a CD4 epitope MP, we further provide a useful tool to detect ex vivo responses of YFV-specific CD4 T cells in small sample volumes.
Details
- Title
- Identification of novel yellow fever Class II epitopes in YF-17D vaccinees
- Authors/Creators
- J. Mateus (Author/Creator)A. Grifoni (Author/Creator)H. Voic (Author/Creator)M.A. Angelo (Author/Creator)E. Phillips (Author/Creator)S. Mallal (Author/Creator)J. Sidney (Author/Creator)A. Sette (Author/Creator)D. Weiskopf (Author/Creator)
- Publication Details
- Viruses, Vol.12(11), Article 1300
- Publisher
- MDPI
- Identifiers
- 991005540945307891
- Copyright
- © 2020 by the authors
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.228 Virology - Tropical Diseases
- 1.228.200 Mosquito-borne Viruses
- Web Of Science research areas
- Virology
- ESI research areas
- Microbiology