Journal article
Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints
Journal of Virological Methods, Vol.165(2), pp.297-301
2010
Abstract
A generic assay to detect and partially characterize unknown viruses from plants was developed. Proteins extracted from virus-infected and uninfected plants were separated in one dimension by SDS polyacrylamide gel electrophoresis. Differentially expressed protein bands were eluted after trypsin digestion and resulting peptide fragments separated according to their mass by matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Resulting peptide mass fingerprints (PMF) were compared with those in protein databases. The assay was used to identify three known viruses: the potyviruses Zucchini yellow mosaic virus and Turnip mosaic virus, and an alfamovirus Alfalfa mosaic virus. It was also used to identify a virus that manifested symptoms in wild Cakile maritima plants, tentatively identified as Pelargonium zonate spot virus (PZSV) (genus Anulavirus) by its PMF, and then confirmed by nucleotide sequencing. The detection of PZSV constitutes a first record of this virus in Australia and in this host. It is proposed that this rapid and simple assay is a useful approach for analysis of plant samples known to harbor viruses that could not be identified using antisera or nucleic acid-based assays.
Details
- Title
- Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints
- Authors/Creators
- H. Luo (Author/Creator) - Murdoch UniversityS.J. Wylie (Author/Creator) - Murdoch UniversityM.G.K. Jones (Author/Creator) - Murdoch University
- Publication Details
- Journal of Virological Methods, Vol.165(2), pp.297-301
- Publisher
- Elsevier BV
- Identifiers
- 991005541041407891
- Copyright
- © 2010 Elsevier B.V.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.267 Virology - Plant
- 3.267.356 Plant Virus Interactions
- Web Of Science research areas
- Biochemical Research Methods
- Biotechnology & Applied Microbiology
- Virology
- ESI research areas
- Microbiology