Identification of polymorphic genes for use in assemblage B genotyping assays through comparative genomics of multiple assemblage B Giardia duodenalis isolates

C. Wielinga; R.C.A. Thompson; P. Monis; U. Ryan

doi:10.1016/j.molbiopara.2015.05.002

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Identification of polymorphic genes for use in assemblage B genotyping assays through comparative genomics of multiple assemblage B Giardia duodenalis isolates

Journal article

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Identification of polymorphic genes for use in assemblage B genotyping assays through comparative genomics of multiple assemblage B Giardia duodenalis isolates

C. Wielinga, R.C.A. Thompson, P. Monis and U. Ryan

Molecular and Biochemical Parasitology, Vol.201(1), pp.1-4

2015

DOI: https://doi.org/10.1016/j.molbiopara.2015.05.002

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Abstract

Giardia duodenalis assemblage B is potentially a zoonotic parasite. The characterisation and investigation of isolates has been hampered by greater genetic diversity of assemblage B, limiting the application and utility of current genotyping loci. Since whole genome sequencing is the optimal high-throughput method for gene identification, the present study sequenced assemblage B isolate BAH15c1 and compared the sequence to the draft GS references to identify polymorphic genes for potential use in genotyping assays. The majority of the genome sequence was conserved between the two isolates, producing 508 contigs of 10.4 Mb with 4968 genes. Seventy polymorphic genes for potential use in genotyping assays were identified ranging in variation from elongation factor 1 α, which was the most conserved, through to triose phosphate isomerase, which was the most variable.

Details

Title: Identification of polymorphic genes for use in assemblage B genotyping assays through comparative genomics of multiple assemblage B Giardia duodenalis isolates
Authors/Creators: C. Wielinga (Author/Creator) - Murdoch University
R.C.A. Thompson (Author/Creator) - Murdoch University
P. Monis (Author/Creator) - South Australian Water Corporation
U. Ryan (Author/Creator) - Murdoch University
Publication Details: Molecular and Biochemical Parasitology, Vol.201(1), pp.1-4
Publisher: Elsevier B.V.
Identifiers: 991005543168407891
Copyright: © 2015 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Biochemistry & Molecular Biology; Parasitology
ESI research areas: Microbiology