Journal article
Identification of potential urine proteins and microRNA biomarkers for the diagnosis of pulmonary tuberculosis patients
Emerging Microbes & Infections, Vol.7(1), pp.1-13
2018
Abstract
This study identified urinary biomarkers for tuberculosis (TB) diagnosis. The urine proteomic profiles of 45 pulmonary tuberculosis patients prior to anti-TB treatment and 45 healthy controls were analyzed and compared using two-dimensional electrophoresis with matrix-assisted laser desorption/ionization time of flight mass spectrometry. Nineteen differentially expressed proteins were identified preliminarily, and western blotting and qRT-PCR were performed to confirm these changes at the translational and transcriptional levels, respectively, using samples from 122 additional pulmonary tuberculosis patients and 73 additional healthy controls. Two proteins, mannose-binding lectin 2 and a 35-kDa fragment of inter-α-trypsin inhibitor H4, exhibited the highest differential expression. We constructed a protein-microRNA interaction network that primarily involved complement and inflammatory responses. Eleven microRNAs from microRNA-target protein interactions were screened and validated using qRT-PCR with some of the above samples, including 97 pulmonary tuberculosis patients and 48 healthy controls. Only miR-625-3p exhibited significant differential expression (p < 0.05). miR-625-3p was increased to a greater extent in samples of smear-positive than smear-negative patients. miR-625-3p was predicted to target mannose-binding lectin 2 protein. A binary logistic regression model based on miR-625-3p, mannose-binding lectin 2, and inter-α-trypsin inhibitor H4 was further established. This three-biomarker combination exhibited better performance for tuberculosis diagnosis than individual biomarkers or any two-biomarker combination and generated a diagnostic sensitivity of 85.87% and a specificity of 87.50%. These novel urine biomarkers may significantly improve tuberculosis diagnosis.
Details
- Title
- Identification of potential urine proteins and microRNA biomarkers for the diagnosis of pulmonary tuberculosis patients
- Authors/Creators
- J. Wang (Author/Creator)X. Zhu (Author/Creator)X. Xiong (Author/Creator)P. Ge (Author/Creator)H. Liu (Author/Creator)N. Ren (Author/Creator)F.A. Khan (Author/Creator)X. Zhou (Author/Creator)L. Zhang (Author/Creator)X. Yuan (Author/Creator)X. Chen (Author/Creator)Y. Chen (Author/Creator)C. Hu (Author/Creator)I.D. Robertson (Author/Creator)H. Chen (Author/Creator)A. Guo (Author/Creator)
- Publication Details
- Emerging Microbes & Infections, Vol.7(1), pp.1-13
- Publisher
- Nature Publishing Group
- Identifiers
- 991005541587907891
- Copyright
- © 2018 The Authors
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.196 Micro & Long Noncoding RNA
- 1.196.68 MicroRNA in Cancer
- Web Of Science research areas
- Immunology
- Infectious Diseases
- Microbiology
- ESI research areas
- Microbiology