Journal article
Identification of the null HLA-A2 allele, A*0232N
Tissue Antigens, Vol.55(1), pp.31-36
2000
Abstract
We have identified a null HLA-A*02 allele, HLA-A*0232N, by using a combination of serology, flow cytometry, polymerase chain reaction using sequence-specific primers (PCR-SSP) and full-length sequencing. The null HLA-A2 allele was identified in an Asian individual originally typed by serology as an apparently homozygous HLA-A3, B51. Subsequent genotyping by PCR-SSP identified the genotype as HLA-A*0201, *0301, B*51, Cw*1402. The serological type and lack of detectable HLA-A2 was confirmed using monoclonal antibody typing reagents. Flow cytometry studies failed to identify any cell surface HLA-A2 expression on the patient 19s peripheral blood lymphocytes. Genotyping using a PCR-SSP set designed to detect null alleles revealed the mutation had not been previously described. Full-length sequencing of the allele identified an allele which was subsequently named HLA-A*0232N. This allele is identical to HLA-A*0201 except for a novel point mutation (T for C) at position 493 which creates a premature stop codon. The sequencing enabled the development of a monospecific A*0232N PCR-SSP reaction which was used to screen 973 DNA samples: no further examples of A*0232N were identified.
Details
- Title
- Identification of the null HLA-A2 allele, A*0232N
- Authors/Creators
- M. Bunce (Author/Creator) - Churchill HospitalJ. Procter (Author/Creator) - Churchill HospitalP.P.J. Dunn (Author/Creator) - United Kingdom Transplant Support Service Authority, Stoke Gifford, Bristol, UKS. Day (Author/Creator) - United Kingdom Transplant Support Service Authority, Stoke Gifford, Bristol, UKJ. Ross (Author/Creator) - United Kingdom Transplant Support Service Authority, Stoke Gifford, Bristol, UKK.I. Welsh (Author/Creator) - Churchill Hospital
- Publication Details
- Tissue Antigens, Vol.55(1), pp.31-36
- Publisher
- Blackwell Publishing
- Identifiers
- 991005542128607891
- Copyright
- 2000 Munksgaard
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.607 MHC Diversity
- Web Of Science research areas
- Cell Biology
- Immunology
- Pathology
- ESI research areas
- Molecular Biology & Genetics