Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L)

L. Ye; Y. Huang; F. Dai; H. Ning; C. Li; M. Zhou; G. Zhang

doi:10.1186/s12864-015-1683-1

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Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L)

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Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L)

L. Ye, Y. Huang, F. Dai, H. Ning, C. Li, M. Zhou and G. Zhang

BMC Genomics, Vol.16(1)

2015

DOI: https://doi.org/10.1186/s12864-015-1683-1

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Abstract

Background In bright beer, haze formation is a serious quality problem, degrading beer quality and reducing its shelf life. The quality of barley (Hordeum vulgare L) malt, as the main raw material for beer brewing, largely affects the colloidal stability of beer. Results In this study, the genetic mechanism of the factors affecting beer haze stability in barley was studied. Quantitative trait loci (QTL) analysis of alcohol chill haze (ACH) in beer was carried out using a Franklin/Yerong double haploid (DH) population. One QTL, named as qACH, was detected for ACH, and it was located on the position of about 108 cM in chromosome 4H and can explain about 20 % of the phenotypic variation. Two key haze active proteins, BATI-CMb and BATI-CMd were identified by proteomics analysis. Bioinformatics analysis showed that BATI-CMb and BATI-CMd had the same position as qACH in the chromosome. It may be deduced that BATI-CMb and BATI-CMd are candidate genes for qACH, controlling colloidal stability of beer. Polymorphism comparison between Yerong and Franklin in the nucleotide and amino acid sequence of BATI-CMb and BATI-CMd detected the corresponding gene specific markers, which could be used in marker-assisted selection for malt barley breeding. Conclusions We identified a novel QTL, qACH controlling chill haze of beer, and two key haze active proteins, BATI-CMb and BATI-CMd. And further analysis showed that BATI-CMb and BATI-CMd might be the candidate genes associated with beer chill haze.

Details

Title: Identification of two key genes controlling chill haze stability of beer in barley (Hordeum vulgare L)
Authors/Creators: L. Ye (Author/Creator) - Zhejiang University
Y. Huang (Author/Creator) - Zhejiang University
F. Dai (Author/Creator) - Zhejiang University
H. Ning (Author/Creator) - Zhejiang University
C. Li (Author/Creator) - Agriculture and Food
M. Zhou (Author/Creator) - University of Tasmania
G. Zhang (Author/Creator) - Zhejiang University
Publication Details: BMC Genomics, Vol.16(1)
Publisher: BioMed Central
Identifiers: 991005539982207891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.220 Smell & Taste Science; 3.220.559 Oenology
Web Of Science research areas: Biotechnology & Applied Microbiology; Genetics & Heredity
ESI research areas: Molecular Biology & Genetics