Journal article
Identification of two novel peanut genotypes resistant to aflatoxin production and their SNP Markers associated with resistance
Toxins, Vol.12(3), Art. 156
2020
Abstract
Aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) are the most common aflatoxins produced by Aspergillus flavus in peanuts, with high carcinogenicity and teratogenicity. Identification of DNA markers associated with resistance to aflatoxin production is likely to offer breeders efficient tools to develop resistant cultivars through molecular breeding. In this study, seeds of 99 accessions of a Chinese peanut mini-mini core collection were investigated for their reaction to aflatoxin production by a laboratory kernel inoculation assay. Two resistant accessions (Zh.h0551 and Zh.h2150) were identified, with their aflatoxin content being 8.11%–18.90% of the susceptible control. The 99 peanut accessions were also genotyped by restriction site-associated DNA sequencing (RAD-Seq) for a genome-wide association study (GWAS). A total of 60 SNP (single nucleotide polymorphism) markers associated with aflatoxin production were detected, and they explained 16.87%–31.70% of phenotypic variation (PVE), with SNP02686 and SNP19994 possessing 31.70% and 28.91% PVE, respectively. Aflatoxin contents of accessions with “AG” (existed in Zh.h0551 and Zh.h2150) and “GG” genotypes of either SNP19994 or SNP02686 were significantly lower than that of “AA” genotypes in the mean value of a three-year assay. The resistant accessions and molecular markers identified in this study are likely to be helpful for deployment in aflatoxin resistance breeding in peanuts.
Details
- Title
- Identification of two novel peanut genotypes resistant to aflatoxin production and their SNP Markers associated with resistance
- Authors/Creators
- B. Yu (Author/Creator) - Oil Crops Research InstituteH. Jiang (Author/Creator) - Oil Crops Research InstituteM.K. Pandey (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsL. Huang (Author/Creator) - Oil Crops Research InstituteD. Huai (Author/Creator) - Oil Crops Research InstituteX. Zhou (Author/Creator) - Oil Crops Research InstituteY. Kang (Author/Creator) - Oil Crops Research InstituteR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsH.K. Sudini (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsX. Ren (Author/Creator) - Oil Crops Research InstituteH. Luo (Author/Creator) - Oil Crops Research InstituteN. Liu (Author/Creator) - Oil Crops Research InstituteW. Chen (Author/Creator) - Oil Crops Research InstituteJ. Guo (Author/Creator) - Oil Crops Research InstituteW. Li (Author/Creator) - Oil Crops Research InstituteY. Ding (Author/Creator) - Oil Crops Research InstituteY. Jiang (Author/Creator) - Oil Crops Research InstituteY. Lei (Author/Creator) - Oil Crops Research InstituteB. Liao (Author/Creator) - Oil Crops Research Institute
- Publication Details
- Toxins, Vol.12(3), Art. 156
- Publisher
- MDPI
- Identifiers
- 991005541291907891
- Copyright
- © 2020 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.198 Mycotoxins
- 3.198.400 Mycotoxin Control
- Web Of Science research areas
- Food Science & Technology
- Toxicology
- ESI research areas
- Pharmacology & Toxicology