Immediate and delayed (after cooling) effects of centrifugation on equine sperm

J. A. Len; J. A. Jenkins; B. E. Eilts; D. L. Paccamonti; S. K. Lyle; G. Hosgood

doi:10.1016/j.theriogenology.2009.09.003

Back

Immediate and delayed (after cooling) effects of centrifugation on equine sperm

Journal article

Peer reviewed

Immediate and delayed (after cooling) effects of centrifugation on equine sperm

J. A. Len, J. A. Jenkins, B. E. Eilts, D. L. Paccamonti, S. K. Lyle and G. Hosgood

Theriogenology, Vol.73(2), pp.225-231

2010

DOI: https://doi.org/10.1016/j.theriogenology.2009.09.003

PMID: 19913898

Files and links (1)

url

PublishedView

Published (Version of Record)

Abstract

Acrosome

Centrifugation

Motility

Plasma membrane

Recovery rate

Sperm

The objectives of this study were to determine the effects of centrifugation on equine sperm total and progressive motility, viability, and acrosomal integrity. We hypothesized that although high centrifugation forces would be detrimental to equine Equus caballus sperm, recovery rates would increase. Ejaculates from six stallions were collected, extended to a concentration of 25 × 106 cells/mL, and subjected for 10 min to (1) no centrifugation (NC) or (2) centrifugation at 400 × g, (3) 900 × g, or (4) 4500 × g. Before and after centrifugation (Day 0), and after 24 h of cooling (Day 1), sperm motility was assessed by computer-assisted semen analysis, and samples were stained with SYBR-14/propidium iodide (PI) for viability and with PI/fluorescein isothiocyanate (FITC)–Peanut aglutinin (PNA) (Arachis hypogaea) for acrosomal integrity. The effect of treatment and day on motility, viability, and acrosomal integrity was determined using a mixed linear model. Compared with the other treatments, centrifugation at 4500 × g reduced all end points measured (P < 0.05). Both 400 × g and 900 × g yielded lower recovery rates than that of 4500 × g (NC = 100.0 ± 0.0%; 400 × g = 54.4 ± 8.6%; 900 × g = 75.0 ± 7.1%; 4500 × g = 97.9 ± 2.8%; P < 0.05). Centrifugation at 400 × g or 900 × g did not damage equine sperm. Based on these findings, further studies of centrifugal forces between 900 × g and 4500 × g are warranted to determine the optimal force that maximizes recovery rate, minimizes sperm damage, and does not affect fertility.

Details

Title: Immediate and delayed (after cooling) effects of centrifugation on equine sperm
Authors/Creators: J. A. Len - Louisiana State University
J. A. Jenkins - Natl Wetlands Res Ctr, US Geol Survey, Lafayette, LA USA
B. E. Eilts - Louisiana State University
D. L. Paccamonti - Louisiana State University
S. K. Lyle - Louisiana State University
G. Hosgood - Louisiana State University
Publication Details: Theriogenology, Vol.73(2), pp.225-231
Publisher: Elsevier
Number of pages: 7
Grant note: Equine Health Study Program, School of Veterinary Medicine, Louisiana State University
Identifiers: 991005592766807891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

34 Record Views

18 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.81 Reproductive Biology; 1.81.176 Male Fertility
Web Of Science research areas: Reproductive Biology; Veterinary Sciences
ESI research areas: Plant & Animal Science