Journal article
Immunization with recombinant beta-tubulin from Trypanosoma evansi induced protection against T. evansi, T. equiperdum and T. b.brucei infection in mice
Parasite Immunology, Vol.29(4), pp.191-199
2007
Abstract
The beta-tubulin gene of Trypanosoma evansi (STIB 806) was cloned and expressed in Escherichia coli. The predicted amino acid sequence of T. evansi beta-tubulin shows 100%, 99·8%, 99·1%, and 98·6% homology with T. equiperdum, T. b. brucei, T. cruzi and T. danilewskyi, respectively, but is diverse from that of T. cyclops, showing only 51·6% of homology. Recombinant beta-tubulin was expressed as inclusion bodies in E. coli. It was purified and renatured for immunological studies. Mice immunized with the renatured recombinant beta-tubulin were protected from lethal challenge with T. evansi STIB 806, T. equiperdum STIB 818 and T. b. brucei STIB 940, showing 83·3%, 70% and 76·7% protection, respectively. Serum collected from the rabbit immunized with recombinant beta-tubulin inhibited the growth of T. evansi, T. equiperdum and T. b. brucei in vitro. Serum from mice and rabbits immunized with recombinant beta-tubulin recognized only T. evansi beta-tubulin and not mouse beta-tubulin. The results of this study demonstrated that the recombinant T. evansi beta-tubulin is a potential candidate for the development of a vaccine to prevent animal trypanosomiasis caused by these three trypanosome species.
Details
- Title
- Immunization with recombinant beta-tubulin from Trypanosoma evansi induced protection against T. evansi, T. equiperdum and T. b.brucei infection in mice
- Authors/Creators
- S-Q Li (Author/Creator) - Sun Yat-sen UniversityM-C Fung (Author/Creator) - Chinese University of Hong KongS.A. Reid (Author/Creator) - Murdoch UniversityN. Inoue (Author/Creator) - Obihiro University of Agriculture and Veterinary MedicineZ-R Lun (Author/Creator) - Sun Yat-sen University
- Publication Details
- Parasite Immunology, Vol.29(4), pp.191-199
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005539592307891
- Copyright
- © 2007 The Authors.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.261 Parasitology - Trypanosoma & Leishmania
- 1.261.596 Trypanosoma Biology
- Web Of Science research areas
- Immunology
- Parasitology
- ESI research areas
- Immunology