Immunohistochemical and clinical evaluation of p53 in canine cutaneous mast cell tumors

M H Jaffe; G Hosgood; H W Taylor; S C Kerwin; C S Hedlund; M K Lopez; J R Davidson; D M Miller; M Paranjpe

doi:10.1354/vp.37-1-40

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Immunohistochemical and clinical evaluation of p53 in canine cutaneous mast cell tumors

Journal article

Peer reviewed

Immunohistochemical and clinical evaluation of p53 in canine cutaneous mast cell tumors

M H Jaffe, G Hosgood, H W Taylor, S C Kerwin, C S Hedlund, M K Lopez, J R Davidson, D M Miller and M Paranjpe

Veterinary pathology, Vol.37(1), pp.40-46

2000

DOI: https://doi.org/10.1354/vp.37-1-40

PMID: 10643979

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Abstract

Immunohistochemistry

mast cell tumor

p53

One hundred twenty-six cutaneous mast cell tumors obtained by excisional biopsy from 106 dogs were evaluated using immunohistochemical staining for the presence of p53 protein. A standard avidin–biotin immunohistochemical protocol was used incorporating a polyclonal antibody of rabbit origin (CM-1) as the primary antibody. Histopathologic grading of tumors was performed on hemotoxylin and eosin–stained samples. There was a significant difference in the percentage of cells staining positive for p53 for the histopathologic grades (P = 0.0005). Grade III tumors had a significantly greater p53 content than did grade I or II tumors (P < 0.05). Clinical data obtained retrospectively was available for 54 dogs. Tumor recurred in 19 of 54 (35.2%) dogs. Twenty-nine dogs died by the end of the study; 9 of 29 (31.0%) died of mast cell tumor disease. Histopathologic grade showed a significant negative association with survival time. Both clinical stage and histopathologic grade showed a significant negative association with time to recurrence. The percentage of cells staining positive for p53 did not significantly improve the forward analysis. Immunohistochemical detection of p53 did not appear useful in characterizing the clinical association between cutaneous mast cell tumor cellular features and survival time or time to tumor recurrence in dogs.

Details

Title: Immunohistochemical and clinical evaluation of p53 in canine cutaneous mast cell tumors
Authors/Creators: M H Jaffe - Louisiana State University
G Hosgood - Louisiana State University
H W Taylor - Louisiana State University
S C Kerwin - Louisiana State University
C S Hedlund - Louisiana State University
M K Lopez
J R Davidson
D M Miller - Georgia College & State University
M Paranjpe - Louisiana State University
Publication Details: Veterinary pathology, Vol.37(1), pp.40-46
Identifiers: 991005592655907891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.232 Veterinary Sciences; 3.232.1120 Veterinary Oncology
Web Of Science research areas: Pathology; Veterinary Sciences
ESI research areas: Plant & Animal Science