Journal article
Immunohistochemical assessment of cyclic guanosine monophosphate (cGMP) and soluble guanylate cyclase (sGC) within the rostral ventrolateral medulla
Journal of Biomedical Science, Vol.15(6), pp.801-812
2008
Abstract
Functional evidence suggests that nitric oxide (NO) signalling in the rostral ventrolateral medulla (RVLM) is cGMP-dependent and that this pathway is impaired in hypertension. We examined cGMP expression as a marker of active NO signalling in the C1 region of the RVLM, comparing adult (>18 weeks) Wistar–Kyoto (WKY, n = 4) and spontaneously hypertensive rats (SHR, n = 4). Double label immunohistochemistry for cGMP-immunoreactivity (IR) and C1 neurons [as identified by phenylethanolamine N-methyltransferase (PNMT-IR) or tyrosine hydroxylase TH-IR)], or neuronal NO synthase (nNOS) neurones, failed to reveal cGMP-IR neurons in the RVLM of either strain, despite consistent detection of cGMP-IR in the nucleus ambiguus (NA). This was unchanged in the presence of isobutylmethylxanthine (IBMX; 0.5 mM, WKY, n = 4, SHR n = 2) and in young animals (WKY, 10-weeks, n = 3). Incubation of RVLM-slices (WKY, 10-weeks, n = 9) in DETA-NO (100 μm; 10 min) or NMDA (10 μM; 2 min) did not uncover cGMP-IR. In all studies, cGMP was prominent within the vasculature. Soluble guanylate cyclase (sGC)-IR was found throughout neurones of the RVLM, but did not co-localise with PNMT, TH or nNOS-IR neurons (WKY, 10-weeks, n = 6). Results indicate that within the RVLM, cGMP is not detectable using immunohistochemistry in the basal state and cannot be elicited by phosphodiesterase inhibition, NMDA receptor stimulation or NO donor application.
Details
- Title
- Immunohistochemical assessment of cyclic guanosine monophosphate (cGMP) and soluble guanylate cyclase (sGC) within the rostral ventrolateral medulla
- Authors/Creators
- K. Powers-Martin (Author/Creator) - Murdoch UniversityA.M. Barron (Author/Creator) - Murdoch UniversityC.H. Auckland (Author/Creator) - Murdoch UniversityJ.K. McCooke (Author/Creator) - Murdoch UniversityD.J. McKitrick (Author/Creator) - The University of Western AustraliaL.F. Arnolda (Author/Creator) - The University of Western AustraliaJ.K. Phillips (Author/Creator) - Murdoch University
- Publication Details
- Journal of Biomedical Science, Vol.15(6), pp.801-812
- Publisher
- Springer
- Identifiers
- 991005544713707891
- Copyright
- 2008 National Science Council Taipei
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.127 Molecular & Cell Biology - Pharmacology
- 1.127.87 Nitric Oxide Roles
- Web Of Science research areas
- Cell Biology
- Medicine, Research & Experimental
- ESI research areas
- Clinical Medicine