Journal article
Immunohistochemical study of the reticular and vascular network of mouse lymph node using vibratome sections
Acta Histochemica, Vol.109(1), pp.15-28
2007
Abstract
The function of lymph nodes is greatly influenced by their unique microanatomy, in which distinct subpopulations of cells are compartmentalized by a meshwork of reticular cells and fibres, specialized blood and lymphatic vessels and nerves. Using antibodies against extracellular matrix (ECM) proteins (fibronectin, collagen IV and laminin), proteoglycan (perlecan), and a fibroblastic marker (ERTR-7), the distribution and molecular organization of the system of reticular fibres was investigated by three-dimensional (3D) reconstruction methods. Fibronectin, collagen IV and laminin are restricted to reticular fibres and have a similar distribution pattern, whereas perlecan is limited to the vascular system of the lymph node. Various compartments of the lymph node, such as the B-cell follicle, paracortex (including the high endothelial venules and paracortical cord), and medulla have been reconstructed to visualize their vasculature with respect to B and T cells. Since the morphology of lymph nodes may change significantly in pathological conditions, different compartments of reactive lymph node (after low-dose Listeria monocytogenes infection), especially germinal centres, were also investigated. The data presented here should facilitate our understanding of the 3D organization of non-immune cell components of lymph nodes, which is crucial for cell adhesion, migration, activation, and differentiation in normal and pathological conditions.
Details
- Title
- Immunohistochemical study of the reticular and vascular network of mouse lymph node using vibratome sections
- Authors/Creators
- B. Ma (Author/Creator)J. Jablonska (Author/Creator)W. Lindenmaier (Author/Creator)K.E.J. Dittmar (Author/Creator)
- Publication Details
- Acta Histochemica, Vol.109(1), pp.15-28
- Publisher
- Elsevier GmbH
- Identifiers
- 991005543275007891
- Copyright
- © 2006 Elsevier GmbH.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.935 Adhesion Molecules
- Web Of Science research areas
- Cell Biology
- ESI research areas
- Biology & Biochemistry