Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat

Dengan Xu; Qianlin Hao; Tingzhi Yang; Xinru Lv; Huimin Qin; Yalin Wang; Chenfei Jia; Wenxing Liu; Xuehuan Dai; Jianbin Zeng; Hongsheng Zhang; Zhonghu He; Xianchun Xia; Shuanghe Cao; Wujun Ma

doi:10.3389/fpls.2023.1147019

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Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat

Journal article

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Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat

Dengan Xu, Qianlin Hao, Tingzhi Yang, Xinru Lv, Huimin Qin, Yalin Wang, Chenfei Jia, Wenxing Liu, Xuehuan Dai, Jianbin Zeng, …

Frontiers in Plant Science, Vol.14, 1147019

2023

DOI: https://doi.org/10.3389/fpls.2023.1147019

PMID: 36938052

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Abstract

coleoptile length

Plant Science

QTL

Rht-B1b

transcriptome

wheat

Wheat coleoptile is a sheath-like structure that helps to deliver the first leaf from embryo to the soil surface. Here, a RIL population consisting of 245 lines derived from Zhou 8425B × Chinese Spring cross was genotyped by the high-density Illumina iSelect 90K assay for coleoptile length (CL) QTL mapping. Three QTL for CL were mapped on chromosomes 2BL, 4BS and 4DS. Of them, two major QTL QCL.qau-4BS and QCL.qau-4DS were detected, which could explain 9.1%–22.2% of the phenotypic variances across environments on Rht-B1 and Rht-D1 loci, respectively. Several studies have reported that Rht-B1b may reduce the length of wheat CL but no study has been carried out at molecular level. In order to verify that the Rht-B1 gene is the functional gene for the 4B QTL, an overexpression line Rht-B1b-OE and a CRISPR/SpCas9 line Rht-B1b-KO were studied. The results showed that Rht-B1b overexpression could reduce the CL, while loss-of-function of Rht-B1b would increase the CL relative to that of the null transgenic plants (TNL). To dissect the underlying regulatory mechanism of Rht-B1b on CL, comparative RNA-Seq was conducted between Rht-B1b-OE and TNL. Transcriptome profiles revealed a few key pathways involving the function of Rht-B1b in coleoptile development, including phytohormones, circadian rhythm and starch and sucrose metabolism. Our findings may facilitate wheat breeding for longer coleoptiles to improve seedling early vigor for better penetration through the soil crust in arid regions.

Details

Title: Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat
Authors/Creators: Dengan Xu - Qingdao Agricultural University
Qianlin Hao - , , , ,
Tingzhi Yang - , , , ,
Xinru Lv - , , , ,
Huimin Qin - , , , ,
Yalin Wang - , , , ,
Chenfei Jia - , , , ,
Wenxing Liu - , , , ,
Xuehuan Dai - , , , ,
Jianbin Zeng - , , , ,
Hongsheng Zhang - , , , ,
Zhonghu He - , , , ,
Xianchun Xia - , , , ,
Shuanghe Cao - , , , ,
Wujun Ma - , , , ,
Publication Details: Frontiers in Plant Science, Vol.14, 1147019
Publisher: Frontiers Media S.A
Identifiers: 991005569359507891
Murdoch Affiliation: Centre for Crop and Food Innovation
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science