Implementation of probes for tracing chromosome segments conferring barley yellow dwarf virus resistance

W. Zhang; M. Carter; S. Matsay; P. Stoutjesdijk; R. Potter; M.G.K. Jones; S. Kleven; R.E. Wilson; P.J. Larkin; M. Turner; K.R. Gale

doi:10.1071/AR01033

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Implementation of probes for tracing chromosome segments conferring barley yellow dwarf virus resistance

Journal article

Peer reviewed

Implementation of probes for tracing chromosome segments conferring barley yellow dwarf virus resistance

W. Zhang, M. Carter, S. Matsay, P. Stoutjesdijk, R. Potter, M.G.K. Jones, S. Kleven, R.E. Wilson, P.J. Larkin, M. Turner, …

Australian Journal of Agricultural Research, Vol.52(11-12), pp.1389-1392

2001

DOI: https://doi.org/10.1071/AR01033

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Abstract

Two PCR-based assays were examined for tracing the presence of a Thinopyrum chromosome segment (Tc6 or Tc14) conferring barley yellow dwarf virus (BYDV) resistance in wheat breeding lines. The microsatellite gwm37 was used to assay the Thinopyrum chromosome segment or its wheat, Group 7, homoeologous segment, and was effective in characterising breeders material since heterozygous lines could be identified. A new set of primers derived from a Thinopyrum-specific DNA segment (csTiB1) provided a dominant marker that was readily scored by agarose gel electrophoresis. It was also demonstrated that the csTiB1 primers could be used to establish a solid phase PCR assay that avoided the requirement for gel electrophoresis and was amenable to use in a high-throughput, microtitre plate format. Depending on the number of DNA samples to be assayed, both primer pairs appear to have a place in breeding programs.

Details

Title: Implementation of probes for tracing chromosome segments conferring barley yellow dwarf virus resistance
Authors/Creators: W. Zhang (Author/Creator)
M. Carter (Author/Creator)
S. Matsay (Author/Creator)
P. Stoutjesdijk (Author/Creator)
R. Potter (Author/Creator)
M.G.K. Jones (Author/Creator)
S. Kleven (Author/Creator)
R.E. Wilson (Author/Creator)
P.J. Larkin (Author/Creator)
M. Turner (Author/Creator)
K.R. Gale (Author/Creator)
Publication Details: Australian Journal of Agricultural Research, Vol.52(11-12), pp.1389-1392
Publisher: CSIRO
Identifiers: 991005540910107891
Copyright: © 2001 CSIRO
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agriculture, Multidisciplinary
ESI research areas: Agricultural Sciences