Journal article
Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars
Plant Cell Reports, Vol.5(1), pp.72-76
1986
Abstract
The regeneration of protoplasts from potato (Solanum tuberosum L.) cvs. Desiree and King Edward has been significantly improved. Different shoot culture media were required for the release of viable protoplasts from cvs. Maris Piper and Desiree, and the response of protoplasts to different culture conditions depended upon the cultivar genotype of the protoplast source. Using protoplast isolation media containing 6mM CaCl2 improved protoplast viability and culture in enriched media lead to the reproducible and relatively efficient recovery of colonies from protoplasts of these cultivars. Over 70% of protoplast-derived calli from King Edward and Desiree regenerated shoots. Many shoots were grown to mature plants in soil. This is the first report of the regeneration of mature Desiree plants from protoplasts.
Details
- Title
- Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars
- Authors/Creators
- D. Foulger (Author/Creator) - Experimental StationM.G.K. Jones (Author/Creator) - Experimental Station
- Publication Details
- Plant Cell Reports, Vol.5(1), pp.72-76
- Publisher
- Springer Verlag
- Identifiers
- 991005542682407891
- Copyright
- © 1986 Springer-Verlag
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.119 Micropropagation
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science