Journal article
Improved isolation of dihaploid Solanum tubersoum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens
In Vitro Cellular & Developmental Biology, Vol.23(8), pp.575-580
1987
Abstract
A modified protoplast isolation technique, applicable to a range of dihaploid Solanum tuberosum genotypes, has been developed. A combination of high calcium and high pH was used to fuse mesophyl protoplasts of dihaploid S. tuberosum (PDH40) and the diploid wild species S. brevidens. Large numbers of colonies were obtained after fusion and putative hybrids selected on the basis of phenotype from regenerated shoots. From these, 11 somatic hybrid plants have been identified by their isoenzyme patterns and morphologic characteristics. Four of these hybrids had the expected chromosome number of 48. The approach of mass culture after fusion followed by selection of hybrids from regenerated shoots and the application of somatic hybridization to potato breeding are discussed.
Details
- Title
- Improved isolation of dihaploid Solanum tubersoum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens
- Authors/Creators
- N. Fish (Author/Creator) - Experimental StationA. Karp (Author/Creator) - Experimental StationM.G.K. Jones (Author/Creator) - Experimental Station
- Publication Details
- In Vitro Cellular & Developmental Biology, Vol.23(8), pp.575-580
- Publisher
- Society for In Vitro Biology
- Identifiers
- 991005540213007891
- Copyright
- © 1987 Tissue Culture Association, Inc.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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