Journal article
Improved spatial resolution of metabolites in tissue biopsies using high-resolution Magic-Angle-Spinning slice localization NMR spectroscopy
Analytical Chemistry, Vol.92(17), pp.11516-11519
2020
Abstract
High-resolution magic-angle-spinning 1H NMR spectroscopy (HR-MAS NMR) is a well-established technique for assessing the biochemical composition of intact tissue samples. In this study, we utilized a method based on HR-MAS NMR spectroscopy with slice localization (SLS) to achieve spatial resolution of metabolites. The obtained 7 slice spectra from each of the model samples (i.e., chicken thigh muscle with skin and murine renal biopsy including medulla (M) and cortex (C)) showed distinct metabolite compositions. Furthermore, we analyzed previously acquired 1H HR-MAS NMR spectra of separated cortex and medulla samples using multivariate statistical methods. Concentrations of glycerophosphocholine (GPC) were found to be significantly higher in the renal medulla compared to the cortex. Using GPC as a biomarker, we identified the tissue slices that were predominantly the cortex or medulla. This study demonstrates that HR-MAS SLS combined with multivariate statistics has the potential for identifying tissue heterogeneity and detailed biochemical characterization of complex tissue samples.
Details
- Title
- Improved spatial resolution of metabolites in tissue biopsies using high-resolution Magic-Angle-Spinning slice localization NMR spectroscopy
- Authors/Creators
- E.V. Vonhof (Author/Creator)M. Piotto (Author/Creator)E. Holmes (Author/Creator)J.C. Lindon (Author/Creator)J.K. Nicholson (Author/Creator)J.V. Li (Author/Creator)
- Publication Details
- Analytical Chemistry, Vol.92(17), pp.11516-11519
- Publisher
- American Chemical Society
- Identifiers
- 991005543389607891
- Copyright
- © 2020 American Chemical Society
- Murdoch Affiliation
- Health Futures Institute; Australian National Phenome Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.211 Mass Spectrometry
- 2.211.990 Metabolomics
- Web Of Science research areas
- Chemistry, Analytical
- ESI research areas
- Chemistry