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Journal article
In Situ Mapping of Nutrient Uptake in the Rhizosphere Using Nanoscale Secondary Ion Mass Spectrometry
Plant physiology (Bethesda), Vol.151(4), pp.1751-1757
2009
PMID: 19812187
Abstract
Plant roots and microorganisms interact and compete for nutrients within the rhizosphere, which is considered one of the most biologically complex systems on Earth. Unraveling the nitrogen (N) cycle is key to understanding and managing nutrient flows in terrestrial ecosystems, yet to date it has proved impossible to analyze and image N transfer in situ within such a complex system at a scale relevant to soil-microbe-plant interactions. Linking the physical heterogeneity of soil to biological processes marks a current frontier in plant and soil sciences. Here we present a new and widely applicable approach that allows imaging of the spatial and temporal dynamics of the stable isotope N-15 assimilated within the rhizosphere. This approach allows visualization and measurement of nutrient resource capture between competing plant cells and microorganisms. For confirmation we show the correlative use of nanoscale secondary ion mass spectrometry, and transmission electron microscopy, to image differential partitioning of (NH4+)-N-15 between plant roots and native soil microbial communities at the submicron scale. It is shown that N-15 compounds can be detected and imaged in situ in individual microorganisms in the soil matrix and intracellularly within the root. Nanoscale secondary ion mass spectrometry has potential to allow the study of assimilatory processes at the submicron level in a wide range of applications involving plants, microorganisms, and animals.
Details
- Title
- In Situ Mapping of Nutrient Uptake in the Rhizosphere Using Nanoscale Secondary Ion Mass Spectrometry
- Authors/Creators
- Peta L. Clode - Centre for Microscopy, Characterisation and AnalysisMatt R. Kilburn - Centre for Microscopy, Characterisation and AnalysisDavid L. Jones - University of WalesElizabeth A. Stockdale - Newcastle Univ, Inst Res Environm & Sustainabil, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, EnglandJohn B. Cliff - Centre for Microscopy, Characterisation and AnalysisAnke M. Herrmann - School of Earth and EnvironmentDaniel V. Murphy - School of Earth and Environment
- Publication Details
- Plant physiology (Bethesda), Vol.151(4), pp.1751-1757
- Publisher
- Amer Soc Plant Biologists
- Number of pages
- 7
- Grant note
- United Kingdom Natural Environmental Research Council; UK Research & Innovation (UKRI); Natural Environment Research Council (NERC) European Commission; European Commission Joint Research Centre NER/G/S/2003/00008 / Natural Environment Research Council; UK Research & Innovation (UKRI); Natural Environment Research Council (NERC) Australian Research Council
- Identifiers
- 991005560421007891
- Copyright
- © 2009 The Author(s).
- Murdoch Affiliation
- Centre for Sustainable Farming Systems
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.45 Soil Science
- 3.45.112 Soil Carbon Dynamics
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science