Journal article
In Vitro Incorporation of LNA Nucleotides
Nucleosides, Nucleotides and Nucleic Acids, Vol.26(8-9), pp.1207-1210
2007
Abstract
An LNA modified nucleoside triphosphate 1 was synthesized in order to investigate its potential to act as substrate for DNA strand synthesis by polymerases. Primer extension assays for the incorporation experiments revealed that Phusion High Fidelity DNA polymerase is an efficient enzyme for incorporation of the LNA nucleotide and for extending strand to full length. It was also observed that pfu DNA polymerase could incorporate the LNA nucleotide but it failed to extend the strand to a full length product.
Details
- Title
- In Vitro Incorporation of LNA Nucleotides
- Authors/Creators
- R.N. Veedu (Author/Creator) - University of Southern DenmarkB. Vester (Author/Creator) - University of Southern DenmarkJ. Wengel (Author/Creator) - University of Southern Denmark
- Publication Details
- Nucleosides, Nucleotides and Nucleic Acids, Vol.26(8-9), pp.1207-1210
- Publisher
- Taylor and Francis
- Identifiers
- 991005541520007891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.170 Nucleic Acids Chemistry
- 2.170.988 Oligonucleotide Modifications
- Web Of Science research areas
- Biochemistry & Molecular Biology
- ESI research areas
- Biology & Biochemistry