In Vitro Susceptibility of Cryptosporidium parvum to Plant Antiparasitic Compounds

S. Ranasinghe; A. Zahedi; A. Armson; A. J. Lymbery; A. Ash

doi:10.3390/pathogens12010061

Back

In Vitro Susceptibility of Cryptosporidium parvum to Plant Antiparasitic Compounds

Journal article

Open access

Peer reviewed

In Vitro Susceptibility of Cryptosporidium parvum to Plant Antiparasitic Compounds

S. Ranasinghe, A. Zahedi, A. Armson, A. J. Lymbery and A. Ash

Pathogens (Basel), Vol.12(1), Art. 61

2022

DOI: https://doi.org/10.3390/pathogens12010061

PMID: 36678409

Files and links (2)

pdf

Published931.67 kBDownload View

CC BY V4.0, Open Access

url

https://www.mdpi.com/2076-0817/12/1/61/pdf?version=1672388708View

Published (Version of Record) Open

Abstract

Ayurveda

cryptosporidiosis

drug screening

cytotoxicity

plant medicine

Cryptosporidium parvum is a significant cause of watery diarrhoea in humans and other animals worldwide. Although hundreds of novel drugs have been evaluated, no effective specific chemotherapeutic intervention for C. parvum has been reported. There has been much recent interest in evaluating plant-derived products in the fight against gastrointestinal parasites, including C. parvum. This study aimed to identify extracts from 13 different plant species that provide evidence for inhibiting the growth of C. parvum in vitro. Efficacy against C. parvum was detected and quantified using quantitative PCR and immunofluorescence assays. All plant extracts tested against C. parvum showed varying inhibition activities in vitro, and none of them produced a cytotoxic effect on HCT-8 cells at concentrations up to 500 µg/mL. Four plant species with the strongest evidence of activity against C. parvum were Curcuma longa, Piper nigrum, Embelia ribes, and Nigella sativa, all with dose-dependent efficacy. To the authors' knowledge, this is the first time that these plant extracts have proven to be experimentally efficacious against C. parvum. These results support further exploration of these plants and their compounds as possible treatments for Cryptosporidium infections.

Details

Title: In Vitro Susceptibility of Cryptosporidium parvum to Plant Antiparasitic Compounds
Authors/Creators: S. Ranasinghe - Murdoch University
A. Zahedi - Centre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Perth 6150, Australia
A. Armson - Psychology, Counselling, Exercise Science and Chiropractic, College of Science, Health, Engineering and Education, Murdoch University, Perth 6150, Australia
A. J. Lymbery - Murdoch University, Centre for Sustainable Aquatic Ecosystems
A. Ash - Murdoch University, Centre for Biosecurity and One Health
Publication Details: Pathogens (Basel), Vol.12(1), Art. 61
Publisher: MDPI
Grant note: RTP Scholarship / Australian Government
Identifiers: 991005548665107891
Murdoch Affiliation: Centre for Sustainable Aquatic Ecosystems; College of Science, Health, Engineering and Education; Harry Butler Institute; Centre for Biosecurity and One Health
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Metrics

29 File views/ downloads

69 Record Views

5 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Microbiology
ESI research areas: Microbiology