Journal article
In Vitro demonstration of targeted phage therapy and competitive exclusion as a novel strategy for decolonization of Extended-Spectrum-Cephalosporin-Resistant Escherichia coli
Applied and Environmental Microbiology, Vol.88(7), pp.Art. aem.02276-21
2022
Abstract
Extended-spectrum cephalosporin-resistant (ESC-R) Escherichia coli have disseminated in food-producing animals globally, attributed to horizontal transmission of blaCTX-M variants, as seen in the InCI1-blaCTX-M-1 plasmid. This ease of transmission, coupled with its demonstrated long-term persistence, presents a significant One Health antimicrobial resistance (AMR) risk. Bacteriophage (phage) therapy is a potential strategy in eliminating ESC-R E. coli in food-producing animals; however, it is hindered by the development of phage-resistant bacteria and phage biosafety concerns. Another alternative to antimicrobials is probiotics, with this study demonstrating that AMR-free commensal E. coli, termed competitive exclusion clones (CECs), can be used to competitively exclude ESC-R E. coli. This study isolated and characterized phages that lysed E. coli clones harboring the InCI1-blaCTX-M-1 plasmid, before investigation of the effect and synergy of phage therapy and competitive exclusion as a novel strategy for decolonizing ESC-resistant E. coli. In vitro testing demonstrated superiority in the combined therapy, reducing and possibly eliminating ESC-R E. coli through phage-mediated lysis coupled with simultaneous prevention of regrowth of phage-resistant mutants due to competitive exclusion with the CEC. Further investigation into this combined therapy in vivo is warranted, with on-farm application possibly reducing ESC-R prevalence, while constricting newly emergent ESC-R E. coli outbreaks prior to their dissemination throughout food-producing animals or humans.
Details
- Title
- In Vitro demonstration of targeted phage therapy and competitive exclusion as a novel strategy for decolonization of Extended-Spectrum-Cephalosporin-Resistant Escherichia coli
- Authors/Creators
- T. Laird (Author/Creator) - Murdoch UniversityR. Abraham (Author/Creator) - Murdoch UniversityS. Sahibzada (Author/Creator) - Murdoch UniversityS. Abraham (Author/Creator) - Murdoch UniversityM. O’Dea (Author/Creator)
- Publication Details
- Applied and Environmental Microbiology, Vol.88(7), pp.Art. aem.02276-21
- Publisher
- American Society for Microbiology
- Identifiers
- 991005542120507891
- Copyright
- © 2022 American Society for Microbiology
- Murdoch Affiliation
- Antimicrobial Resistance and Infectious Disease Laboratory; Harry Butler Institute
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.42 Bacteriology
- 1.42.1349 Bacteriophage
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
- ESI research areas
- Biology & Biochemistry