Journal article
In silico development of simple sequence repeat markers within the aeschynomenoid/dalbergoid and genistoid clades of the Leguminosae family and their transferability to Arachis hypogaea, groundnut
Plant Science, Vol.174(1), pp.51-60
2008
Abstract
Cultivated groundnut (Arachis hypogaea L.) is an agronomically and economically important oilseed crop grown extensively throughout the semi-arid tropics of Asia, Africa and Latin America. The genetic base of the cultivated groundnut is very narrow as a result of the genetic bottleneck associated with recent polyploidization which makes it critical to determine the levels of genetic diversity within available germplasm collections prior to breeding. In groundnut, the use of SSRs for diversity assessment may offer the potential to reveal genetic variation within the genome of the cultivated species. An alternative bioinformatics, or in silico approach, to identifying SSRs suitable for application in cultivated groundnut is presented, as a low-cost alternative to wet lab SSR identification. All available nucleotide sequences from species within the aeschynomenoid/dalbergoid and genistoid clades of the Leguminosae family were searched for SSR motifs and primers designed from 109 unique SSRs. Representative accessions from six genera within the aeschynomenoid/dalbergoid and genistoid clades were selected for assessing SSR-transferability rates. In total, 60% of the total cross-genera transfer testing reactions gave prominent and reproducible amplicons, with 51 of the 109 SSRs amplifying in A. hypogaea. These 51 SSRs were further tested against 27 diverse Arachis accessions and 18 revealed polymorphism, demonstrating that the in silico approach to SSR identification and development is a valid strategy in lesser-studied crops.
Details
- Title
- In silico development of simple sequence repeat markers within the aeschynomenoid/dalbergoid and genistoid clades of the Leguminosae family and their transferability to Arachis hypogaea, groundnut
- Authors/Creators
- E.S. Mace (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsV. Mahalakshmi (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsK. Seetha (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA. Gafoor (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsY. Leeladevi (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsJ.H. Crouch (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
- Publication Details
- Plant Science, Vol.174(1), pp.51-60
- Publisher
- Elsevier
- Identifiers
- 991005540720007891
- Copyright
- © 2007 Elsevier Ireland Ltd.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
31 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science