In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville)

M.D. Bennett; L. Woolford; A.J. O'Hara; K.S. Warren; P.K. Nicholls

doi:10.1099/vir.0.83455-0

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In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville)

Journal article

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In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville)

M.D. Bennett, L. Woolford, A.J. O'Hara, K.S. Warren and P.K. Nicholls

Journal of General Virology, Vol.89(2), pp.419-423

2008

DOI: https://doi.org/10.1099/vir.0.83455-0

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Abstract

The western barred bandicoot (Perameles bougainville) is an endangered Australian marsupial species in which a papillomatosis and carcinomatosis syndrome occurs. Bandicoot papillomatosis carcinomatosis virus type 1 (BPCV1) is associated with the lesions of this progressively debilitating syndrome. Five digoxigenin-labelled DNA probes were generated for in situ hybridization (ISH) and the technique was optimized and performed on formalin-fixed paraffin-embedded (FFPE) biopsies. Staining of keratinoicyte and sebocyte, nuclei within lesions was achieved with all five probes. The sensitivity of ISH (76.9 %) surpassed that of PCR (30.8%) for FFPE samples. The sensitivity of ISH varied from 81 % (papillomas) and 70% (carcinoma in situ) to 29% (squamous cell carcinomas). The specificity of the test was confirmed using an irrelevant probe and papillomas from other species. These results strengthen the association between BPCV1 and the western barred bandicoot papillomatosis and carcinomatosis syndrome and give insight into the biology of the virus-host interaction.

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Title: In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville)
Authors/Creators: M.D. Bennett (Author/Creator) - Murdoch University
L. Woolford (Author/Creator) - Murdoch University
A.J. O'Hara (Author/Creator) - Murdoch University
K.S. Warren (Author/Creator) - Murdoch University
P.K. Nicholls (Author/Creator) - Murdoch University
Publication Details: Journal of General Virology, Vol.89(2), pp.419-423
Publisher: Society for General Microbiology
Identifiers: 991005540070007891
Copyright: © 2008 SGM.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Web Of Science research areas: Biotechnology & Applied Microbiology; Virology
ESI research areas: Microbiology