Journal article
In vitro antioxidant, antiplatelet and anti-inflammatory activity of Carpobrotus rossii (pigface) extract
Journal of Ethnopharmacology, Vol.134(1), pp.97-103
2011
Abstract
Ethnopharmacological relevance
Carpobrotus rossii (CR) has a history of use as a food and therapeutic agent by Australian indigenous peoples and early European settlers and is believed to contain a number of pharmacologically active polyphenolic compounds.
Aims of the study
Oxidation of low density lipoprotein (LDL), platelet aggregation, and inflammation contribute to the development and progression of atherosclerosis. The aim of the present study was to investigate the antioxidant, antiplatelet and anti-inflammatory activity of CR extract using human blood components.
Materials and methods
An assay employing in vitro copper-induced oxidation of serum lipids was used to assess antioxidant activity of CR extract (and tannin, flavonoid and pre- and post-flavonoid fractions). The effects of CR extract on ADP- and collagen-induced platelet aggregation, and on basal (unstimulated) and lipopolysaccharide (LPS)- and phytohaemagglutinin A (PHA)-stimulated cytokine release from peripheral blood mononuclear cells (PBMC) were also investigated.
Results
CR extract increased the lag time of serum oxidation (maximum of ∼4-fold at 20 μg/ml) in a concentration-dependent manner. The antioxidant activity resided only in the tannin and post-flavonoid fractions. CR had no effect on ADP-induced platelet aggregation, but significantly decreased collagen-induced platelet aggregation. LPS, but not PHA, significantly increased the release of IL-1β and TNF-α from PBMC. CR extract alone inhibited monocyte chemoattractant protein (MCP)-1 release and in the presence of LPS, inhibited IL-10, TNF-α and MCP-1 release compared to LPS alone.
Conclusion
CR has significant in vitro antioxidant, antiplatelet and, potentially, anti-inflammatory activity.
Details
- Title
- In vitro antioxidant, antiplatelet and anti-inflammatory activity of Carpobrotus rossii (pigface) extract
- Authors/Creators
- D.P. Geraghty (Author/Creator) - University of TasmaniaK.D.K. Ahuja (Author/Creator) - University of TasmaniaJ. Pittaway (Author/Creator) - University of TasmaniaC. Shing (Author/Creator) - University of TasmaniaG.A. Jacobson (Author/Creator) - University of TasmaniaN. Jager (Author/Creator) - University of TasmaniaS. Jurković (Author/Creator) - University of TasmaniaC. Narkowicz (Author/Creator) - University of TasmaniaC.I. Saunders (Author/Creator) - University of TasmaniaM. Ball (Author/Creator) - University of TasmaniaA. Pinkard (Author/Creator) - University of TasmaniaR.R. Vennavaram (Author/Creator) - University of TasmaniaM.J. Adams (Author/Creator) - University of Tasmania
- Publication Details
- Journal of Ethnopharmacology, Vol.134(1), pp.97-103
- Publisher
- Elsevier
- Identifiers
- 991005542242607891
- Copyright
- © 2010 Elsevier Ireland Ltd
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.16 Phytochemicals
- 3.16.28 Antioxidant Activity
- Web Of Science research areas
- Chemistry, Medicinal
- Integrative & Complementary Medicine
- Pharmacology & Pharmacy
- Plant Sciences
- ESI research areas
- Pharmacology & Toxicology