Journal article
In vitro cultivation and fruit body formation of the black bolete, Phlebopus portentosus, a popular edible ectomycorrhizal fungus in Thailand
Mycoscience, Vol.51(1), pp.15-22
2010
Abstract
The tropical black bolete Phlebopus portentosus is highly favored in the cuisine of northern Thailand. It is suspected to form ectomycorrhizae with many host trees. Mycelium of P. portentosus isolated from a basidiome in Chiang Rai Province in 2003 grew well on modified Gamborg, modified Melin-Norkans, and Murashige and Skoog media at 30°C and at pH 4. In vitro fructification of P. portentosus on sorghum grain medium without a host plant is presented for the first time. Basidiomes emerged 3 months after inoculation on the medium, and the produced basidiospores germinated on agar, indicating the completion of its life cycle in vitro without a host. Five putative host plants (Castanopsis tribuloides, Dipterocarpus alatus, Dimocarpus longan, Pinus kesiya, and Syzygium cumini) were inoculated with mycelium on sorghum grain medium in a greenhouse to confirm its ectomycorrhizal status. Ectomycorrhizal roots were observed only on Pinus kesiya, suggesting that P. portentosus may be facultatively ectomycorrhizal. Identification of the synthesized ectomycorrhizae was confirmed by PCR amplification of ITS with a designed specific primer (HAR2A).
Details
- Title
- In vitro cultivation and fruit body formation of the black bolete, Phlebopus portentosus, a popular edible ectomycorrhizal fungus in Thailand
- Authors/Creators
- R. Sanmee (Author/Creator) - Chiang Mai UniversityP. Lumyong (Author/Creator) - Chiang Mai UniversityB. Dell (Author/Creator) - Murdoch UniversityS. Lumyong (Author/Creator) - Chiang Mai University
- Publication Details
- Mycoscience, Vol.51(1), pp.15-22
- Publisher
- Springer Verlag
- Identifiers
- 991005541505607891
- Copyright
- © The Mycological Society of Japan and Springer 2010.
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.488 Mycorrhizal Symbiosis
- Web Of Science research areas
- Mycology
- ESI research areas
- Plant & Animal Science