Journal article
In vitro culture and non-invasive metabolic profiling of single bovine embryos
Reproduction, Fertility and Development, Vol.31(2), pp.306-314
2019
Abstract
Selecting high-quality embryos for transfer has been a difficult task when producing bovine embryos in vitro. The most used non-invasive method is based on visual observation. Molecular characterisation of embryo growth media has been proposed as a complementary method. In this study we demonstrate a culture medium sampling method for identifying potential embryonic viability markers to predict normal or abnormal embryonic development. During single embryo culture, 20 µL culture media was removed at Days 2, 5 and 8 after fertilisation from the same droplet (60 µL). In all, 58 samples were analysed using liquid chromatography–mass spectrometry. We demonstrate that it is possible to remove samples from the same culture medium droplets and not significantly affect blastocyst rate (25.2%). Changes in any single low molecular weight compound were not predictive enough. Combining multiple low molecular weight signals made it possible to predict Day 2 and 5 embryo development to the blastocyst stage with an accuracy of 64%. Elevated concentrations of lysophosphatidylethanolamines (m/z = 453, 566, 588) in the culture media of Day 8 well-developing embryos were observed. Choline (104 m/z) and citrate (215 m/z) concentrations were increased in embryos in which development was retarded. Metabolic profiling provides possibilities to identify well-developing embryos before transfer, thus improving pregnancy rates and the number of calves born.
Details
- Title
- In vitro culture and non-invasive metabolic profiling of single bovine embryos
- Authors/Creators
- M. Nõmm (Author/Creator) - Estonian University of Life SciencesR. Porosk (Author/Creator) - University of TartuP. Pärn (Author/Creator) - Estonian University of Life SciencesK. Kilk (Author/Creator) - University of TartuU. Soomets (Author/Creator) - University of TartuS. Kõks (Author/Creator) - Estonian University of Life SciencesÜ. Jaakma (Author/Creator) - Estonian University of Life Sciences
- Publication Details
- Reproduction, Fertility and Development, Vol.31(2), pp.306-314
- Publisher
- CSIRO Publishing
- Identifiers
- 991005544855707891
- Copyright
- © 2019 CSIRO
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.339 Embryo Development
- Web Of Science research areas
- Developmental Biology
- Reproductive Biology
- Zoology
- ESI research areas
- Plant & Animal Science