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Journal article
In vitro effect of carprofen and meloxicam on the conductance and permeability to mannitol and the histologic appearance of the gastric mucosa of dogs
American Journal of Veterinary Research, Vol.72(4), pp.570-577
2011
Abstract
Objective
To evaluate the effects of carprofen and meloxicam on conductance and permeability to mannitol and on the histologic appearance of sections of canine gastric mucosa.
Sample
Gastric mucosa from 6 mature mixed-breed dogs.
Procedures
Sections of gastric mucosa were mounted in Ussing chambers, and carprofen (40 or 400μg/mL [CAR40 and CAR400, respectively]), meloxicam (8 or 80μg/mL [MEL8 and MEL80, respectively]), or no drug (controls) was added to the bathing solution. For all sections, conductance was calculated every 15 minutes for 240 minutes and flux of mannitol was calculated for 3 consecutive 1-hour periods; histologic examination was performed after the experiment. The area under the conductance-time curve for each chamber was calculated. Values of conductance × time, flux of mannitol, and the frequency distribution of histologic findings were analyzed for treatment effects.
Results
For CAR400- and MEL80-treated sections, conductance X time was significantly higher than that for control and MEL8-treated sections. The effect of CAR40 treatment was not different from that of any other treatment. Over the three 1-hour periods, mannitol flux increased significantly in MEL80-, CAR40-, and CAR400-treated sections but not in MEL8- treated or control sections. Major histologic changes including epithelial cell sloughing were limited to the CAR400-treated sections.
Conclusions and Clinical Relevance
In the gastric mucosa of dogs, carprofen and meloxicam increased in vitro conductance and permeability to mannitol. At a concentration of 400 μg/mL, carprofen caused sloughing of epithelial cells. Carprofen and meloxicam appear to compromise gastric mucosal integrity and barrier function in dogs.
Details
- Title
- In vitro effect of carprofen and meloxicam on the conductance and permeability to mannitol and the histologic appearance of the gastric mucosa of dogs
- Authors/Creators
- M.A. Hicks (Author/Creator)G.L. Hosgood (Author/Creator)T.W. Morgan (Author/Creator)C.A. Briere (Author/Creator)R.S. McConnico (Author/Creator)
- Publication Details
- American Journal of Veterinary Research, Vol.72(4), pp.570-577
- Publisher
- American Veterinary Medical Association
- Identifiers
- 991005544568207891
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences; School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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