In vitro evaluation of the contractile response to endothelin-1 of the circular and longitudinal myometrial layers of the uterine horn of nongravid mares

Honor A Walesby; Changaram S Venugopal; Giselle Hosgood; Susan C Eades; Rustin M Moore

doi:10.2460/ajvr.2005.66.1094

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In vitro evaluation of the contractile response to endothelin-1 of the circular and longitudinal myometrial layers of the uterine horn of nongravid mares

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In vitro evaluation of the contractile response to endothelin-1 of the circular and longitudinal myometrial layers of the uterine horn of nongravid mares

Honor A Walesby, Changaram S Venugopal, Giselle Hosgood, Susan C Eades and Rustin M Moore

American journal of veterinary research, Vol.66(6), pp.1094-1100

2005

DOI: https://doi.org/10.2460/ajvr.2005.66.1094

PMID: 16008237

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Abstract

Objective To characterize the in vitro response of circular and longitudinal myometrial layers of the uterine horn (CMLH and LMLH, respectively) of horses to endothelin (ET)-1 by use of specific ETA (BQ-123) and ETB (IRL-1038) receptor antagonists. Sample Population Uteruses from 10 nongravid mares in anestrus. Procedure Muscle strips from the CMLH and LMLH were suspended in tissue baths and connected to force-displacement transducers interfaced with a polygraph. Strips were incubated for 45-minute intervals with no antagonist (control specimens), and 3 concentrations (10–9, 10–7, and 10–5M) of BQ-123, IRL- 1038, or BQ-123 and IRL-1038 before concentration response curves to ET-1 were generated. Contractile response to cumulative concentrations of ET-1 (10–9 to 10–6M) was quantified by measuring change in the area under the curve (AUC) for the 3-minute period after each ET-1 dose. Results ET-1 caused concentration-dependent contraction of the CMLH and LMLH specimens. Application of BQ-123 decreased AUC values for both layers. Application of IRL-1038 increased the AUC value for LMLH specimens but did not affect the CMLH value. The combination of BQ-123 and IRL-1038 decreased the AUC value for LMLH tissue and increased that for CMLH tissue. Conclusions and Clinical Relevance ET-1 causes contraction of the CMLH and LMLH in nongravid horses. In both layers, ETA receptors mediate contraction but the role of ETB receptors remains unclear. In the LMLH, ETA receptors have a dominant role; the presence of another receptor or receptor subtype within this layer is suggested. These findings support a physiologic role for ET-1 in uterine contractility.

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Title: In vitro evaluation of the contractile response to endothelin-1 of the circular and longitudinal myometrial layers of the uterine horn of nongravid mares
Authors/Creators: Honor A Walesby
Changaram S Venugopal
Giselle Hosgood
Susan C Eades
Rustin M Moore
Publication Details: American journal of veterinary research, Vol.66(6), pp.1094-1100
Identifiers: 991005591573007891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.127 Molecular & Cell Biology - Pharmacology; 1.127.1303 Endothelin
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science