In vitro isolation and characterisation of the first canine Neospora caninum isolate in Australia

L.M. McInnes; P. Irwin; D.G. Palmer; U.M. Ryan

doi:10.1016/j.vetpar.2006.01.018

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In vitro isolation and characterisation of the first canine Neospora caninum isolate in Australia

Journal article

Peer reviewed

In vitro isolation and characterisation of the first canine Neospora caninum isolate in Australia

L.M. McInnes, P. Irwin, D.G. Palmer and U.M. Ryan

Veterinary Parasitology, Vol.137(3-4), pp.355-363

30/04/2006

DOI: https://doi.org/10.1016/j.vetpar.2006.01.018

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Abstract

Neospora caninum was isolated and established in vitro from the skin lesion of a naturally infected dog. The identity of the parasite was evaluated by immunofluorescent antibody test (IFAT), microscopy, Western blotting and polymerase chain reaction (PCR). N. caninum DNA was detected in the whole blood, serum, skin lesion, rectal scrapings and faeces of the infected dog utilising a nested PCR targeting the Nc-5 gene of N. caninum. Antigenic and genetic characterisation of the isolate, designated WA-K9, at a number of loci including the Nc-5 gene, heat shock protein 70 (HSP-70) gene, alpha-tubulin and beta-tubulin genes revealed no variation between this isolate and two N. caninum isolates from different geographic areas. Clinical aspects of this case, which included cutaneous and neurological disease, are also discussed.

Details

Title: In vitro isolation and characterisation of the first canine Neospora caninum isolate in Australia
Authors/Creators: L.M. McInnes (Author/Creator) - Murdoch University
P. Irwin (Author/Creator) - Murdoch University
D.G. Palmer (Author/Creator) - Animal Health Laboratories, Department of Agriculture Western Australia, South Perth, WA 6151, Australia
U.M. Ryan (Author/Creator) - Murdoch University
Publication Details: Veterinary Parasitology, Vol.137(3-4), pp.355-363
Publisher: Elsevier BV
Identifiers: 991005539995407891
Copyright: © 2006 Elsevier B.V. All rights reserved.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.217 Parasitology - Malaria, Toxoplasmosis & Coccidiosis; 1.217.1038 Toxoplasma Gondii
Web Of Science research areas: Parasitology; Veterinary Sciences
ESI research areas: Plant & Animal Science