In vitro responses of equine colonic arterial and venous rings to adenosine triphosphate

Joanne Tetens; Changaram S. Venugopal; Earnestine P. Holmes; Catherine E. Koch; Giselle Hosgood; Rustin M. Moore

doi:10.2460/ajvr.2001.62.1928

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In vitro responses of equine colonic arterial and venous rings to adenosine triphosphate

Journal article

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Peer reviewed

In vitro responses of equine colonic arterial and venous rings to adenosine triphosphate

Joanne Tetens, Changaram S. Venugopal, Earnestine P. Holmes, Catherine E. Koch, Giselle Hosgood and Rustin M. Moore

American journal of veterinary research, Vol.62(12), pp.1928-1933

2001

DOI: https://doi.org/10.2460/ajvr.2001.62.1928

PMID: 11763183

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Abstract

Objective To evaluate the in vitro effects of adenosine tryphosphate (ATP) on vasomotor tone of equine colonic vasculature. Sample Population Arteries and veins from the left ventral colon of 14 mixed-breed horses euthanatized for reasons unrelated to cardiovascular or gastrointestinal tract disease. Procedures Endothelium-intact and -denuded arterial and venous rings were precontracted with 10–7 and 1.8 × 10–8 M endothelin-1, respectively. In 1 trial, endothelium-intact rings were also incubated with 10–4 M Nω-nitro-L-arginine methyl ester (L-NAME) to inhibit nitric oxide (NO) production. Adenosine triphosphate (10–8 to 10–3 M) was added in a noncumulative manner, and relaxation percentage versus time curves were generated. Areas under the curves (ie, percentage of relaxation time) were calculated. Results Relaxation response of arterial and venous rings to ATP was dose-dependent. Percentage of relaxation time in response to 10–4 and 10–3 M ATP was significantly greater, compared with that for rings not treated with ATP. Removal of endothelium attenuated but did not eliminate the relaxation response. Addition of L-NAME did not attenuate the relaxation response in arteries. At higher concentrations, the vascular response to ATP was biphasic. Conclusions and Clinical Relevance ATP applied to equine colonic arterial and venous rings with and without intact endothelium induced a biphasic response characterized by transient contraction followed by slow, substantial, and sustained relaxation. This ATP-induced response is possibly mediated by a mechanism other than NO. Adenosine triphosphate may be a useful treatment to modulate colonic vasomotor tone in horses with strangulating volvulus of the ascending colon.

Details

Title: In vitro responses of equine colonic arterial and venous rings to adenosine triphosphate
Authors/Creators: Joanne Tetens - Louisiana State University
Changaram S. Venugopal
Earnestine P. Holmes
Catherine E. Koch
Giselle Hosgood
Rustin M. Moore
Publication Details: American journal of veterinary research, Vol.62(12), pp.1928-1933
Identifiers: 991005592648407891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.159 Membrane Channels & Receptors; 1.159.576 Adenosine Signaling
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science