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Published (Version of Record)CC BY-NC V4.0, Open Access
Journal article
In vitro susceptibility of Pythium insidiosum and a Lagenidium sp to itraconazole, posaconazole, voriconazole, terbinafine, caspofungin, and mefenoxam
American journal of veterinary research, Vol.69(11), pp.1463-1468
2008
PMID: 18980428
Abstract
Objective
To evaluate the radial growth assay for use in in vitro susceptibility testing of Pythium insidiosum and a Lagenidium sp and to assess susceptibility of representative isolates to itraconazole, posaconazole, voriconazole, terbinafine, caspofungin, and mefenoxam.
Sample Population
6 isolates each of P insidiosum and Lagenidium sp.
Procedures
Isolates were plated in triplicate onto agar supplemented with antifungal compounds at concentrations of 0.025 to 8 μg/mL. Isolates on dimethyl sulfoxide– and water-supplemented agar served as control samples. Effect of antifungal concentration on colony diameter was assessed with a mixed linear model. Assay variability was assessed with the coefficient of variation.
Results
Colony growth was uniform (mean intra-assay and interassay coefficients of variation were < 5%). Minimal inhibition was evident with voriconazole and posaconazole at 8 μg/mL. Terbinafine at 8 μg/mL significantly reduced growth of P insidiosum and at ≥ 1 μg/mL significantly reduced growth of the Lagenidium sp. Caspofungin and mefenoxam (concentrations ≥ 1 μg/mL and ≥ 0.025 μg/mL, respectively) significantly reduced growth of both pathogens. Mefenoxam at 0.1 μg/mL caused > 50% growth inhibition in 11 of 12 isolates and at 1 μg/mL caused > 90% inhibition in all isolates.
Conclusions and Clinical Relevance
Results suggested that the radial growth assay was a simple, reproducible technique for susceptibility testing of P insidiosum and a Lagenidium sp. Azoles had limited activity, whereas terbinafine and caspofungin caused significant but minimal to moderate inhibition. Only mefenoxam had a profound effect on both pathogens at concentrations likely to be achievable in tissues.
Details
- Title
- In vitro susceptibility of Pythium insidiosum and a Lagenidium sp to itraconazole, posaconazole, voriconazole, terbinafine, caspofungin, and mefenoxam
- Authors/Creators
- Tina A. Brown - Louisiana State Univ, Sch Vet Med, Dept Vet Clin Sci, Baton Rouge, LA 70803 USAAmy M. Grooters - Louisiana State Univ, Sch Vet Med, Dept Vet Clin Sci, Baton Rouge, LA 70803 USAGiselle L. Hosgood - Louisiana State Univ, Sch Vet Med, Dept Vet Clin Sci, Baton Rouge, LA 70803 USA
- Publication Details
- American journal of veterinary research, Vol.69(11), pp.1463-1468
- Publisher
- Amer Veterinary Medical Assoc
- Number of pages
- 6
- Identifiers
- 991005591572807891
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.1439 Phytophthora Management
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science