Journal article
In vivo evaluation of intra-articular protection in a novel model of canine cranial cruciate ligament mid-substance elongation injury
Veterinary surgery, Vol.35(8), pp.711-720
Submitted February 2006; Accepted June 2006
2006
PMCID: PMC1899127
PMID: 17187633
Abstract
Objectives
To evaluate the effects of intra-articular protection (IAP) on the canine cranial cruciate ligament (CrCL) and stifle in a CrCL midsubstance elongation injury model.
Study Design
Experimental longitudinal cohort study.
Animals
Skeletally mature female mixed breed hounds (n=12; mean±SEM weight, 25.6±0.7 kg).
Methods
After CrCL elongation in 1 stifle of each dog, IAP was applied in 6 joints. In vivo assessment included radiographs, cranial–caudal joint translation, gait analysis, and synovial fluid levels of 3B3(−) (proteoglycan epitope) and C2C (collagen II neoepitope) up to 12 weeks after surgery. Joint translation and rotation were quantified at necropsy. CrCL midsubstance length was determined before and after elongation and at necropsy. CrCLs were subjectively assessed with light microscopy. Comparisons were made between stifles containing elongated CrCLs with and without IAP and unoperated controls.
Results
Four weeks after surgery, ground reaction forces were significantly decreased in operated limbs. Absolute C2C levels were significantly elevated in operated stifles 4 weeks post-surgery. C2C and 3B3(−) levels normalized to total protein were significantly elevated in IAP+ stifles 8 weeks after surgery. Protected CrCLs appeared to have decreased granulation tissue and better collagen fiber alignment.
Conclusions
IAP has negligible effects on the canine stifle based on the response variables evaluated in this 12-week study. Protection of elongated CrCLs may promote reduced, organized scar formation.
Clinical Relevance
These results support the healing capacity of the canine CrCL midsubstance following elongation injury and IAP application to potentially reduce cicatrix formation in elongated CrCLs.
Details
- Title
- In vivo evaluation of intra-articular protection in a novel model of canine cranial cruciate ligament mid-substance elongation injury
- Authors/Creators
- MANDI J. Lopez - Louisiana State UniversitySANDRA O. Robinson - University of Wisconsin–MadisonMARGARET M. Quinn - Louisiana State UniversityGISELLE Hosgood - Louisiana State UniversityMARK D. Markel - Louisiana State University
- Publication Details
- Veterinary surgery, Vol.35(8), pp.711-720
- Publisher
- Blackwell Publishing Inc
- Edition
- Submitted February 2006; Accepted June 2006
- Number of pages
- 10
- Identifiers
- 991005592770007891
- Copyright
- © 2006 by The American College of Veterinary Surgeons
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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25 Record Views
InCites Highlights
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.34 Orthopedics
- 1.34.440 Anterior Cruciate Ligament
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science