Increased placental expression and maternal serum levels of apoptosis-inducing TRAIL in recurrent miscarriage

K. Rull; K. Tomberg; S. Kõks; J. Männik; M. Möls; M. Sirotkina; S. Värv; M. Laan

doi:10.1016/j.placenta.2012.11.032

Back

Increased placental expression and maternal serum levels of apoptosis-inducing TRAIL in recurrent miscarriage

Journal article

Peer reviewed

Increased placental expression and maternal serum levels of apoptosis-inducing TRAIL in recurrent miscarriage

K. Rull, K. Tomberg, S. Kõks, J. Männik, M. Möls, M. Sirotkina, S. Värv and M. Laan

Placenta, Vol.34(2), pp.141-148

2013

DOI: https://doi.org/10.1016/j.placenta.2012.11.032

Files and links (1)

url

Link to Published Version *Subscription may be requiredView

Abstract

Introduction Recurrent miscarriage (RM; ≥3 consecutive pregnancy losses) occurs in 1–3% of fertile couples. No biomarkers with high predictive value of threatening miscarriage have been identified. We aimed to profile whole-genome differential gene expression in RM placental tissue, and to determine the protein levels of identified loci in maternal sera in early pregnancy. Methods GeneChips (Affymetrix®) were used for discovery and Taqman RT-qPCR assays for replication of mRNA expression in placentas from RM cases (n = 13) compared to uncomplicated pregnancies matched for gestational age (n = 23). Concentrations of soluble TRAIL (sTRAIL) and calprotectin in maternal serum in normal first trimester (n = 35) and failed pregnancies (early miscarriage, n = 18, late miscarriage, n = 4; tubal pregnancy, n = 11) were determined using ELISA. Results In RM placentas 30 differentially expressed (with nominal P-value < 0.05) transcripts were identified. Significantly increased placental mRNA expression of TNF-related apoptosis-inducing ligand (TRAIL; P = 1.4 × 10−3; fold-change 1.68) and S100A8 (P = 7.9 × 10−4; fold-change 2.56) encoding for inflammatory marker calprotectin (S100A8/A9) was confirmed by RT-qPCR. When compared to normal first trimester pregnancy (sTRAIL 16.1 ± 1.6 pg/ml), significantly higher maternal serum concentration of sTRAIL was detected at the RM event (33.6 ± 4.3 pg/ml, P = 0.00027), and in pregnant women, who developed an unpredicted miscarriage 2–50 days after prospective serum sampling (28.5 ± 4.4 pg/ml, P = 0.039). Women with tubal pregnancy also exhibited elevated sTRAIL (30.5 ± 3.9 pg/ml, P = 0.035). Maternal serum levels of calprotectin were neither diagnostic nor prognostic to early pregnancy failures (P > 0.05). Conclusions The study indicated of sTRAIL as a potential predictive biomarker in maternal serum for early pregnancy complications.

Details

Title: Increased placental expression and maternal serum levels of apoptosis-inducing TRAIL in recurrent miscarriage
Authors/Creators: K. Rull (Author/Creator) - University of Tartu
K. Tomberg (Author/Creator) - University of Tartu
S. Kõks (Author/Creator) - University of Tartu
J. Männik (Author/Creator) - University of Tartu
M. Möls (Author/Creator) - University of Tartu
M. Sirotkina (Author/Creator) - Tartu University Hospital
S. Värv (Author/Creator) - University of Tartu
M. Laan (Author/Creator) - University of Tartu
Publication Details: Placenta, Vol.34(2), pp.141-148
Publisher: Elsevier
Identifiers: 991005541419007891
Copyright: © 2012 Elsevier Ltd.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

32 Record Views

48 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Citation topics: 1 Clinical & Life Sciences; 1.81 Reproductive Biology; 1.81.1272 Reproductive Immunology
Web Of Science research areas: Developmental Biology; Obstetrics & Gynecology; Reproductive Biology
ESI research areas: Clinical Medicine