Journal article
Increased wheat protein content via introgression of an HMW glutenin selectively reshapes the grain proteome
Molecular & Cellular Proteomics, Vol.20, Article 100097
2021
Abstract
Introgression of a high-molecular-weight glutenin subunit (HMW-GS) allele, 1Ay21∗, into commercial wheat cultivars increased overall grain protein content and bread-making quality, but the role of proteins beyond this HMW-GS itself was unknown. In addition to increased abundance of 1Ay HMW-GS, 115 differentially accumulated proteins (DAPs) were discovered between three cultivars and corresponding introgressed near-isogenic lines. Functional category analysis showed that the DAPs were predominantly other storage proteins and proteins involved in protein synthesis, protein folding, protein degradation, stress response, and grain development. Nearly half the genes encoding the DAPs showed strong coexpression patterns during grain development. Promoters of these genes are enriched in elements associated with transcription initiation and light response, indicating a potential connection between these cis-elements and grain protein accumulation. A model of how this HMW-GS enhances the abundance of machinery for protein synthesis and maturation during grain filling is proposed. This analysis not only provides insights into how introgression of the 1Ay21∗ improves grain protein content but also directs selection of protein candidates for future wheat quality breeding programs.
Details
- Title
- Increased wheat protein content via introgression of an HMW glutenin selectively reshapes the grain proteome
- Authors/Creators
- H. Cao (Author/Creator) - The University of Western AustraliaO. Duncan (Author/Creator) - The University of Western AustraliaS. Islam (Author/Creator) - Murdoch UniversityJ. Zhang (Author/Creator) - Murdoch UniversityW. Ma (Author/Creator) - Murdoch UniversityA.H. Millar (Author/Creator) - The University of Western Australia
- Publication Details
- Molecular & Cellular Proteomics, Vol.20, Article 100097
- Publisher
- Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology
- Identifiers
- 991005541864007891
- Copyright
- © 2021 The Authors
- Murdoch Affiliation
- College of Science, Health, Engineering and Education; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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