Journal article
Industrial control of recombinant E. coli fed-batch culture: new perspectives on traditional controlled variables
Bioprocess and Biosystems Engineering, Vol.25(2), pp.111-120
2002
Abstract
Three industrially relevant fed-batch algorithms were applied to the production of recombinant proteins in Escherichia coli BL21 DE3. Starvation dissolved-oxygen (DO)-transient control sustained growth rates greater than those in pH-stat (0.16 h-1 versus 0.11-0.13 h-1) while feed-up DO-transient control better tracked the measured threshold for acetate production (ยต~0.2 h-1). All controllers supported growth without acetate production, resulting in end concentrations of recombinant protein up to 20 times greater than in batch culture. Both DO-transient control systems were judged superior to pH-stat for their ability to detect and track the acetate threshold. Results also showed that although high cell density at the stationary phase is desirable, this parameter may be dictated by the choice of media and reactor design as opposed to controller type. Controller selection, however, has a great impact on the capacity to track the acetate threshold and therefore to enhance productivity without concomitant acetate production.
Details
- Title
- Industrial control of recombinant E. coli fed-batch culture: new perspectives on traditional controlled variables
- Authors/Creators
- W. Johnston (Author/Creator) - Murdoch UniversityR. Cord-Ruwisch (Author/Creator) - Murdoch UniversityM.J. Cooney (Author/Creator) - Murdoch University
- Publication Details
- Bioprocess and Biosystems Engineering, Vol.25(2), pp.111-120
- Publisher
- Springer
- Identifiers
- 991005544313307891
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology; School of Engineering
- Language
- English
- Resource Type
- Journal article
- Publisher URL
- http://www.springer.com/chemistry/biotechnology/journal/449
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.873 Protein Purification
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Engineering, Chemical
- ESI research areas
- Engineering